MED13 Antibody

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MED13 antibody (NBP3-48617) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

MED13 Antibody Summary

• Description: Novus Biologicals Rabbit MED13 Antibody (NBP3-48617) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human MED13. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MED13
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for MED13 Antibody

• Activator-recruited cofactor 250 kDa component
• KIAA0593HSPC221
• mediator complex subunit 13ARC250
• mediator of RNA polymerase II transcription, subunit 13 homolog
• THRAP1mediator of RNA polymerase II transcription subunit 13
• thyroid hormone receptor associated protein 1
• Thyroid hormone receptor-associated protein 1
• Thyroid hormone receptor-associated protein complex 240 kDa component
• thyroid hormone receptor-associated protein complex component TRAP240
• thyroid hormone receptor-associated protein, 240 kDa subunit
• Trap240
• TRAP240DRIP250
• Vitamin D3 receptor-interacting protein complex component DRIP250

Background

MED13 encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. The product of this gene is proposed to form a sub-complex with MED12, cyclin C, and CDK8 that can negatively regulate transactivation by mediator.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MED13