MED13 Antibody

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Western Blot: MED13 Antibody [NB100-60641] - Detection of Human THRAP1/TRAP240 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified ...read more
Western Blot: MED13 Antibody [NB100-60641] - Detection of Human THRAP1/TRAP240 on HeLa whole cell lysate using NB100-60641. THRAP1/TRAP240 was also immunoprecipitated by rabbit anti-THRAP1/TRAP240 antibody NB100-60642.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

MED13 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 325 and 375 of human thyroid hormone receptor associated protein 1 (thyroid hormone receptor-associated protein, 240 kDa) using the numbering given in entry NP_005112.2 (GeneID 996
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MED13
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunoprecipitation 1:10-1:500

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MED13 Antibody

  • Activator-recruited cofactor 250 kDa component
  • KIAA0593HSPC221
  • mediator complex subunit 13ARC250
  • mediator of RNA polymerase II transcription, subunit 13 homolog
  • THRAP1mediator of RNA polymerase II transcription subunit 13
  • thyroid hormone receptor associated protein 1
  • Thyroid hormone receptor-associated protein 1
  • Thyroid hormone receptor-associated protein complex 240 kDa component
  • thyroid hormone receptor-associated protein complex component TRAP240
  • thyroid hormone receptor-associated protein, 240 kDa subunit
  • Trap240
  • TRAP240DRIP250
  • Vitamin D3 receptor-interacting protein complex component DRIP250

Background

This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. The product of this gene is proposed to form a sub-complex with MED12, cyclin C, and CDK8 that can negatively regulate transactivation by mediator.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Additional MED13 Products

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Bioinformatics

Gene Symbol MED13
Entrez
Uniprot