MED13 Antibody

Western Blot: MED13 Antibody [NB100-60641] - Detection of Human THRAP1/TRAP240 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-THRAP1/TRAP240 antibody NB100-60641 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

MED13 Antibody [NB100-60641] - Detection of human THRAP1/TRAP240 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-THRAP1/TRAP240 antibody A301-277A (lot NB100-60641-2) used for IP at 6 ug per reaction. THRAP1/TRAP240 was also immunoprecipitated by a previous lot of this antibody (lot NB100-60641-1) and another rabbit anti-THRAP1/TRAP240 antibody. For blotting immunoprecipitated THRAP1/TRAP240, NB100-60641 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

MED13 Antibody Summary

• Description: Novus Biologicals Rabbit MED13 Antibody (NB100-60641) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 325 and 375 of human thyroid hormone receptor associated protein 1 (thyroid hormone receptor-associated protein, 240 kDa) using the numbering given in entry NP_005112.2 (GeneID 996
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MED13
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MED13 Antibody

• Activator-recruited cofactor 250 kDa component
• KIAA0593HSPC221
• mediator complex subunit 13ARC250
• mediator of RNA polymerase II transcription, subunit 13 homolog
• THRAP1mediator of RNA polymerase II transcription subunit 13
• thyroid hormone receptor associated protein 1
• Thyroid hormone receptor-associated protein 1
• Thyroid hormone receptor-associated protein complex 240 kDa component
• thyroid hormone receptor-associated protein complex component TRAP240
• thyroid hormone receptor-associated protein, 240 kDa subunit
• Trap240
• TRAP240DRIP250
• Vitamin D3 receptor-interacting protein complex component DRIP250

Background

This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. The product of this gene is proposed to form a sub-complex with MED12, cyclin C, and CDK8 that can negatively regulate transactivation by mediator.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MED13
• Entrez:
  • 9969(Human)
• Uniprot:
  • NP_005112.2(Human)