MeCP2 Antibody - BSA Free

Western Blot: MeCP2 Antibody [NBP3-05542] - Western blot analysis of tissue and cell lysates using MeCP2 Antibody at a dilution of 1:20,000 in green: [1] protein standard (red), [2] rat whole brain, [3] nuclear fraction of rat brain, [4] mouse whole brain, [5] nuclear fraction of mouse brain lysate, [6] C6 cell lysate, and [7] SH-SY5Y cell lysate. The strong band at about 75kDa corresponds to the MeCP2 protein. The MeCP2 proteins of rat and human origin are known to migrate slightly differently on SDS-PAGE gels compared to that of mouse, and as a result bands appear at somewhat different apparent molecular weights.

Immunocytochemistry/Immunofluorescence: MeCP2 Antibody [NBP3-05542] - Immunofluorescent analysis of rat olfactory bulb section stained with chicken pAb to MeCP2 at a dilution of 1:2,000 in red and costained with mouse mAb to alpha-synuclein at a dilution of 1:1,000, in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with the above antibodies. The MeCP2 antibody specifically labels the nuclei of neuronal cells while the alpha-synuclein antibody reveals alpha-synuclein protein concentrated in presynaptic regions.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Chicken
• Conjugate: Unconjugated
• Format: BSA Free

MeCP2 Antibody - BSA Free Summary

• Description: Novus Biologicals Chicken MeCP2 Antibody - BSA Free (NBP3-05542) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant MeCP2 expressed in and purified from E. coli.
• Isotype: IgY
• Clonality: Polyclonal
• Host: Chicken
• Gene: MECP2
• Purity: IgY purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:1000-1:2000
  • Immunohistochemistry 1:1000-1:2000
  • Western Blot 1:10000-1:20000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Supplied as concentrated total IgY preparation from egg yolk. Exact concentration of target specific IgY is not quantifiable as the preparation contains both immune IgY specific for the target and also irrelevant, non-immune IgY.
• Preservative: 0.02% Sodium Azide
• Purity: IgY purified

Notes

Chicken products cannot be exported to Canada.

Alternate Names for MeCP2 Antibody - BSA Free

• AUTSX3
• DKFZp686A24160
• MeCp-2 protein
• mental retardation, X-linked 79
• methyl CpG binding protein 2 (Rett syndrome)
• X-linked 16

Background

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of some cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MECP2