MDR1/ABCB1 Antibody (UIC2)

Product Discontinued

MDR1/ABCB1 Antibody (UIC2) Summary

• Immunogen: Mouse Balb/c 3T3 fibroblasts transfected with human CD243 cDNA
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ABCB1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50 - 1:100
  • Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
• Application Notes: Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. A suggested positive control for immunohistochemical applications is human kidney. For Flow analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for MDR1/ABCB1 Antibody (UIC2)

• ABC20
• ABCB1
• ABCB1B
• ATP-binding cassette sub-family B member 1
• ATP-binding cassette, sub-family B (MDR/TAP), member 1
• CD243 antigen
• CD243
• CLCS
• colchicin sensitivity
• doxorubicin resistance
• EC 3.6.3
• EC 3.6.3.44
• GP170
• IBD13
• MDR1
• MDR1MGC163296
• multidrug resistance protein 1
• P-glycoprotein 1
• P-gp
• PGY1
• PGY1P-GP

Background

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for MDR1/ABCB1 Antibody (NBP1-43787). (Showing 1 - 1 of 1 FAQ).

1. Can this product (UIC2) be used in the presence of inhibitor P-gp on MCF-7/dox-res cell lines?
   • Unfortunately we have not tested this antibody in the presence of the inhibitor P-gp.

Bioinformatics

• Gene Symbol: ABCB1