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MDR1/ABCB1 Antibody (MRK16) [DyLight 350]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, ICC/IF
Clone
MRK16
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 350

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-52701AF350
Alexa Fluor 405
NBP2-52701AF405
Alexa Fluor 488
NBP2-52701AF488
Alexa Fluor 532
NBP2-52701AF532
Alexa Fluor 594
NBP2-52701AF594
Alexa Fluor 647
NBP2-52701AF647
Alexa Fluor 700
NBP2-52701AF700
Alexa Fluor 750
NBP2-52701AF750
Allophycocyanin
NBP2-52701APC
DyLight 405
NBP2-52701V
DyLight 488
NBP2-52701G
DyLight 550
NBP2-52701R
DyLight 650
NBP2-52701C
DyLight 680
NBP2-52701FR
DyLight 755
NBP2-52701IR
FITC
NBP2-52701F
Janelia Fluor 549
NBP2-52701JF549
Janelia Fluor 646
NBP2-52701JF646
PE
NBP2-52701PE
PerCP
NBP2-52701PCP
Unconjugated
NBP2-52701-0.1mg
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MDR1/ABCB1 Antibody (MRK16) [DyLight 350] Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Drug resistant K-562 cells.
Specificity
Recognizes a discontinuous extracellular epitope on P-glycoprotein.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ABCB1
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for MDR1/ABCB1 Antibody (MRK16) [DyLight 350]

  • ABC20
  • ABCB1
  • ABCB1B
  • ATP-binding cassette sub-family B member 1
  • ATP-binding cassette, sub-family B (MDR/TAP), member 1
  • CD243 antigen
  • CD243
  • CLCS
  • colchicin sensitivity
  • doxorubicin resistance
  • EC 3.6.3
  • EC 3.6.3.44
  • GP170
  • IBD13
  • MDR1
  • MDR1MGC163296
  • multidrug resistance protein 1
  • P-glycoprotein 1
  • P-gp
  • PGY1
  • PGY1P-GP

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Dual RNAscope ISH-IHC: MRP1 Antibody (IU2H10) [NB400-156] - Formalin-fixed paraffin-embedded tissue sections of human testis were probed for MRP1 mRNA (ACD RNAScope Probe, catalog # 435591; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using mouse monoclonal (Novus Biologicals catalog # NB400-156) at 7ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to Leydig cells and seminiferous tubule cells.
Immunocytochemistry/Immunofluorescence: MRP1 Antibody (IU2H10) [NB400-156] - The MRP-1 antibody was tested in HeLa cells at a 1:50 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.
Western Blot: MRP1 Antibody (IU2H10) [NB400-156] - Vector-transfected HEK293 cells 2: MRP1-transfected cells, prep1 3: MRP1-transfected cells, prep2 (the lower band is a degradation product.)
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Immunocytochemistry/Immunofluorescence: ABCG2/CD338 Antibody (3G8) [NBP2-22124] - Analysis of Hela cells using ABCG2 mouse mAb (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).
Immunohistochemistry-Paraffin: ABCG2/CD338 Antibody (3G8) [NBP2-22124] - Analysis of paraffin-embedded bladder cancer tissues (left) and skeletal muscle tissues (right) using ABCG2 mouse mAb with DAB staining.
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Immunohistochemistry-Paraffin: MRP2 Antibody (JA32-01) [NBP2-66958] - Analysis of paraffin-embedded human liver tissue using anti-MRP2 antibody. Counter stained with hematoxylin.
Flow Cytometry: MRP2 Antibody (JA32-01) [NBP2-66958] - Analysis of A549 cells with MRP2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
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Immunocytochemistry/Immunofluorescence: MSH3 Antibody [NBP2-19417] - MSH3 antibody [C2C3], C-term detects MSH3 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: MSH3 stained by MSH3 antibody [C2C3], C-term diluted at 1:500.Blue: Fluoroshield with DAPI.Scale bar= 10 um.
Immunohistochemistry-Paraffin: MSH3 Antibody [NBP2-19417] - MSH3 antibody [C2C3], C-term detects MSH3 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.MSH3 stained by MSH3 antibody [C2C3], C-term diluted at 1:600.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Cytochrome P450
3A4 Antibody (3H8)
NBP2-37502
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NBP2-58327
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MARCKS like
protein Antibody (8C8
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Immunohistochemistry: LRP-1 Antibody (A2Mr alpha-2) [NB100-64808] - Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD91 antibody, clone A2Mr alpha-2 followed by the Histar detection system. Low power.
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Product General Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-52701AF350
Alexa Fluor 405 NBP2-52701AF405
Alexa Fluor 488 NBP2-52701AF488
Alexa Fluor 532 NBP2-52701AF532
Alexa Fluor 594 NBP2-52701AF594
Alexa Fluor 647 NBP2-52701AF647
Alexa Fluor 700 NBP2-52701AF700
Alexa Fluor 750 NBP2-52701AF750
Allophycocyanin NBP2-52701APC
DyLight 350 NBP2-52701UV
DyLight 405 NBP2-52701V
DyLight 488 NBP2-52701G
DyLight 550 NBP2-52701R
DyLight 650 NBP2-52701C
DyLight 680 NBP2-52701FR
DyLight 755 NBP2-52701IR
FITC NBP2-52701F
Janelia Fluor 549 NBP2-52701JF549
Janelia Fluor 646 NBP2-52701JF646
PE NBP2-52701PE
PerCP NBP2-52701PCP
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol ABCB1