MDR1/ABCB1 Antibody (JSB-1)

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Product Details

Summary
Reactivity Hu, Ha, Mu(-), Rt(-)Species Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro
Clone
JSB-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

MDR1/ABCB1 Antibody (JSB-1) Summary

Immunogen
The antibody reacts with P Glycoprotein.
Specificity
The antibody JSB-1 reacts with a conserved cytoplasmic epitope of the plasma membrane-associated 170-180 kD glycoprotein, the expression of which is strongly correlated with the degree of multi-drug-resistance (MDR) derived MDR cell lines and human MDR cell lines, including cell lines derived from lung, ovaries and B cell lymphomas. cross reacts with chinese hamster. No cross reactivity with mouse or rat.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ABCB1
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10
  • Flow Cytometry 1:10
  • Immunocytochemistry/Immunofluorescence 1:20
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:20
  • Immunohistochemistry-Paraffin 1:20
  • In vitro assay
Application Notes
JSB-1 has potential value for diagnostic detection of MDR cells in human tumor samples. Optimal staining results are obtained with routine 2-step ABC or APAAP methods using acetone-fixed cytocentrifuge preparations or cryostat sections. In vitro assay (PMID: 21889999)
Publications
Read Publication using NBP1-51768.

Reactivity Notes

Human, Hamster, Mouse (-), Rat (-)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.25 ml concentrated tissue culture supernatant with 0.25 mg/ml immunoglobulin and in 1.0% BSA
Preservative
0.1% Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for MDR1/ABCB1 Antibody (JSB-1)

  • ABC20
  • ABCB1
  • ABCB1B
  • ATP-binding cassette sub-family B member 1
  • ATP-binding cassette, sub-family B (MDR/TAP), member 1
  • CD243 antigen
  • CD243
  • CLCS
  • colchicin sensitivity
  • doxorubicin resistance
  • EC 3.6.3
  • EC 3.6.3.44
  • GP170
  • IBD13
  • MDR1
  • MDR1MGC163296
  • multidrug resistance protein 1
  • P-glycoprotein 1
  • P-gp
  • PGY1
  • PGY1P-GP

Background

This monoclonal antibody reacts with human Multidrug Resistant (MDR)-1, which is also known as P-glycoprotein (Pgp) and CD243. A 170-kDa transmembrane protein, MDR-1 is an ATP-dependent efflux pump for lipophilic compounds, including anti-cancer drugs. Expression of MDR-1 has been shown to correlate with multidrug resistance. In fact, tumor resistance to chemotherapy has been linked to MDR-1 expression in many cancers. MDR-1 is expressed in a variety of tissues, including the brain, kidney, liver, pancreas, and testes. Within the immune system, this molecule can be found on normal T, B, and natural killer cells, but not on monocytes. This antibody has been reported to inhibit MDR1-mediated efflux.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ABCB1
Entrez
Uniprot