MDC1 Antibody

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Independent Antibodies: Western Blot: MDC1 Antibody [NB100-397] - Detection of Human MDC1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP ...read more
Immunohistochemistry-Paraffin: MDC1 Antibody [NB100-397] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MDC1

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

       

Order Details

MDC1 Antibody Summary

Immunogen
The immunogen this antibody was made to, maps to a region between residues 1375 and 1425 of human Mediator of DNA Damage Checkpoint 1 using the numbering given in entry NP_055456.1 (GeneID 9656).
Predicted Species
Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MDC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:5000-1:25000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. ICC/IF reactivity reported in (PMID: 22373579).
Publications
Read Publications using
NB100-397 in the following applications:

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Chimpanzee.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MDC1 Antibody

  • DKFZp781A0122
  • Em:AB023051.5
  • KIAA0170MGC166888
  • MDC1
  • mediator of DNA damage checkpoint 1
  • mediator of DNA damage checkpoint protein 1
  • mediator of DNA-damage checkpoint 1
  • NFBD1
  • NFBD1homologue to Drosophila photoreceptor protein calphotin
  • Nuclear factor with BRCT domains 1

Background

MDC1 (mediator of DNA damage checkpoint protein 1), which works with H2AX to promote recruitment of repair proteins to the sites of DNA breaks and controls damage-induced cell-cycle arrest checkpoints. Cells that lack MDC1 are sensitive to ionizing radiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-397
Species: Hu, Pm
Applications: WB, ICC/IF, IHC, IHC-P, IP

Publications for MDC1 Antibody (NB100-397)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.


Filter By Application
ICC/IF
(2)
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Filter By Species
Human
(2)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MDC1 Antibody (NB100-397) (0)

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Secondary Antibodies

 

Isotype Controls

Additional MDC1 Products

Array NB100-397

Bioinformatics Tool for MDC1 Antibody (NB100-397)

Discover related pathways, diseases and genes to MDC1 Antibody (NB100-397). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for MDC1 Antibody (NB100-397)

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Pathways for MDC1 Antibody (NB100-397)

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Research Areas for MDC1 Antibody (NB100-397)

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Blogs on MDC1.

Further unraveling the role of gamma H2AX in DNA damage response
Our genome experiences a moderate amount of DNA damage in our cells on a daily basis.  This DNA damage can be in response to external environmental factors, or be a result of our internal metabolic processes going awry.  While normal rates of DNA ...  Read full blog post.

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Bioinformatics

Gene Symbol MDC1
Entrez
Uniprot