MCM3 Antibody (4F7)

Western Blot: MCM3 Antibody (4F7) [H00004172-M01] - MCM3 monoclonal antibody (M01), clone 4F7. Analysis of MCM3 expression in human colon.

Immunocytochemistry/Immunofluorescence: MCM3 Antibody (4F7) [H00004172-M01] - Analysis of monoclonal antibody to MCM3 on HeLa cell. Antibody concentration 10 ug/ml.

Western Blot: MCM3 Antibody (4F7) [H00004172-M01] - MCM3 monoclonal antibody (M01), clone 4F7. Analysis of MCM3 expression in HeLa.

Western Blot: MCM3 Antibody (4F7) [H00004172-M01] - MCM3 monoclonal antibody (M01), clone 4F7 Analysis of MCM3 expression in COLO 320 HSR.

Western Blot: MCM3 Antibody (4F7) [H00004172-M01] - MCM3 monoclonal antibody (M01), clone 4F7. Analysis of MCM3 expression in Raw 264.7.

Western Blot: MCM3 Antibody (4F7) [H00004172-M01] - MCM3 monoclonal antibody (M01), clone 4F7. Analysis of MCM3 expression in A-431.

Western Blot: MCM3 Antibody (4F7) [H00004172-M01] - MCM3 monoclonal antibody (M01), clone 4F7. Analysis of MCM3 expression in Daoy.

Western Blot: MCM3 Antibody (4F7) [H00004172-M01] - MCM3 monoclonal antibody (M01), clone 4F7. Analysis of MCM3 expression in K-562.

Sandwich ELISA: MCM3 Antibody (4F7) [H00004172-M01] - Detection limit for recombinant GST tagged MCM3 is 0.1 ng/ml as a capture antibody.

Product Discontinued

MCM3 Antibody (4F7) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: MCM3 (NP_002379, 699 a.a. ~ 808 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AKDGDSYDPYDFSDTEEEMPQVHTPKTADSQETKESQKVELSESRLKAFKVALLDVFREAHAQSIGMNRLTESINRDSEEPFSSVEIQAALSKMQDDNQVMVSEGIIFLI
• Specificity: MCM3 - MCM3 minichromosome maintenance deficient 3 (S. cerevisiae) (4F7)
• Isotype: IgG2a Lambda
• Clonality: Monoclonal
• Host: Mouse
• Gene: MCM3
• Purity: Ascites

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate, tissue lysate and recombinant protein for WB. It has been used for IF and ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Ascites

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MCM3 Antibody (4F7)

• cervical cancer proto-oncogene 5
• DNA polymerase alpha holoenzyme-associated protein P1
• DNA replication factor MCM3
• DNA replication licensing factor MCM3
• EC 3.6.4.12
• HCC5
• hRlf beta subunit
• MCM3 minichromosome maintenance deficient 3 (S. cerevisiae)
• MCM3 minichromosome maintenance deficient 3
• MGC1157
• minichromosome maintenance complex component 3
• minichromosome maintenance deficient (S. cerevisiae) 3
• minichromosome maintenance deficient 3
• P1.h
• p102
• P1-MCM3
• replication licensing factor, beta subunit
• RLF subunit beta
• RLFB

Background

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MCM3
• OMIM:
  • 602693(Human)