MCM2 Antibody

Western Blot: MCM2 Antibody [NBP2-17238] - Sample (30 ug of whole cell lysate) A: HCT116 7. 5% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: MCM2 Antibody [NBP2-17238] - HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MCM2 protein stained by MCM2 antibody [N1N2], N-term diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50.

Immunoprecipitation: MCM2 Antibody [NBP2-17238] - IP samples: HCT-116 whole cell extract A. 40 ug HCT-116 whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of MCM2 protein by 4 ug MCM2 antibody [N1N2], N-term 7.5 % SDS-PAGE The immunoprecipitated MCM2 protein was detected by MCM2 antibody [N1N2], N-term diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]

Product Discontinued

MCM2 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human MCM2. The exact sequence is proprietary.
• Predicted Species: Mouse (94%), Rat (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MCM2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 102 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (81%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for MCM2 Antibody

• BM28
• BM28MGC10606
• CCNL 1
• CCNL1
• cdc19
• CDCL1
• CDCL1mitotin
• D3S3194
• EC 3.6.4.12
• KIAA0030MITOTIN
• MCM2 minichromosome maintenance deficient 2, mitotin (S. cerevisiae)
• MCM2
• minichromosome maintenance complex component 2
• minichromosome maintenance deficient (S. cerevisiae) 2 (mitotin)
• minichromosome maintenance deficient 2 (mitotin)
• Mitotin

Background

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: MCM2
• Uniprot:
  • P49736()