MCM2 Antibody

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Western Blot: MCM2 Antibody [NB100-244] - Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified goat anti-MCM2 antibody used for WB at 0.5 ug/ml. Detection: ...read more
Western Blot: MCM2 Antibody [NB100-244] - Whole cell lysate (60 ug) from HeLa cells. Antibody used at 0.25 ug/ml.
Proximity Ligation Assay: MCM2 Antibody [NB100-244] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MCM2 (NB100-244) and rabbit anti-human ATM (NB100-678). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: MCM2 Antibody [NB100-244] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MCM2 (NB100-244) and rabbit anti-human CPSF160 (NB100-79822). Image merged from DAPI (2ms), ...read more
Proximity Ligation Assay: MCM2 Antibody [NB100-244] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MCM2 (NB100-244) and rabbit anti-human DBF4 (NB100-88114). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: MCM2 Antibody [NB100-244] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MCM2 (NB100-244) and rabbit anti-human HBO (NBP1-26609). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: MCM2 Antibody [NB100-244] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MCM2 (NB100-244) and rabbit anti-human HIF1-alpha (NB100-449). Image merged from DAPI (2ms) and ...read more
Proximity Ligation Assay: MCM2 Antibody [NB100-244] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MCM2 (NB100-244) and rabbit anti-huam phospho-MCM2 [S108] (NB100-639). Image merged from DAPI ...read more
Proximity Ligation Assay: MCM2 Antibody [NB100-244] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MCM2 (NB100-244) and rabbit anti-human MCPH1 (NB100-575). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: MCM2 Antibody [NB100-244] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MCM2 (NB100-244) and rabbit anti-human ORC1 (NBP1-05957). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: MCM2 Antibody [NB100-244] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MCM2 (NB100-244) and rabbit anti-human ORC2 (NBP1-46175). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: MCM2 Antibody [NB100-244] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MCM2 (NB100-244) and rabbit anti-human p16INK4A (NB100-77315). Image merged from DAPI (2ms) and ...read more
Proximity Ligation Assay: MCM2 Antibody [NB100-244] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MCM2 (NB100-244) and rabbit anti-human RPA2 (NB100-332). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: MCM2 Antibody [NB100-244] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MCM2 (NB100-244) and rabbit anti-human phospho-RPA2 [S33] (NB100-544). Image merged from DAPI ...read more
Proximity Ligation Assay: MCM2 Antibody [NB100-244] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MCM2 (NB100-244) and rabbit anti-human phospho-RPA2 [S4/S8] (NBP1-23017). Image merged from DAPI ...read more
Proximity Ligation Assay: MCM2 Antibody [NB100-244] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MCM2 (NB100-244) and rabbit anti-human Timeless (NB100-40852). Image merged from DAPI (2ms) and ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PLA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

MCM2 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human Minichromosomal Maintenance Deficient 2 using the numbering given in entry NP_0045117.2 (GeneID 4171).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MCM2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Proximity Ligation Assay 1:1000-1:20000
Application Notes
By Western blot, this antibody recognizes bands around 130 kDa representing MCM2. *The investigator should determine the optimal working dilution for a specific application.
Positive Control
HeLa Lysate (NB800-PC1)
MCM2 Lysate (NBL1-12950)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MCM2 Antibody

  • BM28
  • BM28MGC10606
  • CCNL 1
  • CCNL1
  • cdc19
  • CDCL1
  • CDCL1mitotin
  • D3S3194
  • EC 3.6.4.12
  • KIAA0030MITOTIN
  • MCM2 minichromosome maintenance deficient 2, mitotin (S. cerevisiae)
  • MCM2
  • minichromosome maintenance complex component 2
  • minichromosome maintenance deficient (S. cerevisiae) 2 (mitotin)
  • minichromosome maintenance deficient 2 (mitotin)
  • Mitotin

Background

The MCM (Mini-Chromosome Maintenance) complex is a key component of the pre-replication complex involved in replication licensing which restricts DNA replication to only once per cell cycle. The MCM complex is a heterohexamer of MCM2, MCM3, MCM4, MCM5, MCM6, and MCM7. MCM2 interacts with the core MCM complex (MCM4, 6, and 7) to regulate its helicase activity. Alternate names for MCM2 include DNA replication licensing factor MCM2, nuclear protein BM28, CCNL1, CDCL1, MITOTIN, and KIAA0030.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for MCM2 Antibody (NB100-244)

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Bioinformatics

Gene Symbol MCM2
Entrez