MCM2 Antibody [Unconjugated]

Western blot shows lysates of Ramos human Burkitt's lymphoma cell line and DA3 mouse myeloma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse MCM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5778) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for MCM2 at approximately 125kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

MCM2 was detected in immersion fixed paraffin-embedded sections of human colon array using Goat Anti-Human/Mouse MCM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5778) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

MCM2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human MCM2
  Asp143-Ser296
  Accession # P49736
• Specificity: Detects human and mouse MCM2 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MCM2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MCM2 Antibody [Unconjugated]

• BM28
• BM28MGC10606
• CCNL 1
• CCNL1
• cdc19
• CDCL1
• CDCL1mitotin
• D3S3194
• EC 3.6.4.12
• KIAA0030MITOTIN
• MCM2 minichromosome maintenance deficient 2, mitotin (S. cerevisiae)
• MCM2
• minichromosome maintenance complex component 2
• minichromosome maintenance deficient (S. cerevisiae) 2 (mitotin)
• minichromosome maintenance deficient 2 (mitotin)
• Mitotin

Background

CM2 (Minichromosome maintenance protein 2; also BM28) is a member of the MCM family of proteins. Although its predicted MW is 102 kDa, it runs anomalously at 125 kDa in SDS‑PAGE. MCM2 forms a large, 450‑600 kDa prereplication complex with MCM3 through 7 plus HBO1, and associates with the ORC to initiate DNA synthesis. Human MCM2 is 904 amino acids (aa) in length (Accession # P49736). It contains one C4‑type zinc finger region (aa 329‑355) and a P‑loop NTPase region (aa 485‑659) and at least 12 Ser/Thr phosphorylation sites in the N‑terminal one‑third of the molecule. MCM2 has several potential isoforms. There is an alternate start site at Met10 with or without a 47 aa substitution for aa 109‑157, a deletion of aa 31‑126, a Gly substitution for aa 113‑132 coupled with a 69 aa insertion after Lys476, and a seven aa substitution for aa 1‑137. Over aa 143‑296, human MCM2 shares 99% aa identity with mouse MCM2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MCM2 Antibody (AF5778)(3)

Publications using AF5778

• Foucault, L;Capeliez, T;Angonin, D;Lentini, C;Bezin, L;Heinrich, C;Parras, C;Donega, V;Marcy, G;Raineteau, O; Neonatal brain injury unravels transcriptional and signaling changes underlying the reactivation of cortical progenitors Cell reports 2024-02-12 [PMID: 38349790] (Immunocytochemistry, Mouse, Transgenic Mouse)
• Q Zheng, X Dai, W Fang, Y Zheng, J Zhang, Y Liu, D Gu Overexpression of microRNA-367 inhibits angiogenesis in ovarian cancer by downregulating the expression of LPA1 Cancer Cell Int, 2020-10-02;20(0):476. 2020-10-02 [PMID: 33024414] (Western Blot, Human)
• AJ Crowther, SA Lim, B Asrican, BH Albright, J Wooten, CY Yeh, H Bao, DH Cerri, J Hu, YY Ian Shih, A Asokan, J Song An Adeno-Associated Virus-Based Toolkit for Preferential Targeting and Manipulating Quiescent Neural Stem Cells in the Adult Hippocampus Stem Cell Reports, 2018-03-01;10(3):1146-1159. 2018-03-01 [PMID: 29478897] (IHC, Mouse)

Bioinformatics

• Gene Symbol: MCM2
• Uniprot:
  • P49736()