MCM2 Antibody (MCM2/8006R) - Azide and BSA Free Summary
Description |
Positive Controls: MCF-7, Jurkat or Ramos cells. Human tonsil.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required. |
Additional Information |
Recombinant Monoclonal Antibody |
Immunogen |
Recombinant fragment (aa 1-200) of human MCM2 protein (exact sequence is proprietary) |
Localization |
Nucleus. |
Isotype |
IgG Kappa |
Clonality |
Monoclonal |
Host |
Rabbit |
Gene |
MCM2 |
Purity |
Protein A or G purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunohistochemistry-Paraffin 1-2 ug/ml
|
Application Notes |
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined. |
Packaging, Storage & Formulations
Storage |
Store at -20 to -70C. Avoid freeze-thaw cycles. |
Buffer |
10mM PBS |
Preservative |
No Preservative |
Concentration |
1 mg/ml |
Purity |
Protein A or G purified |
Alternate Names for MCM2 Antibody (MCM2/8006R) - Azide and BSA Free
Background
MCM2 is encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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