MCM2 Antibody (2B3)

Western Blot: MCM2 Antibody (2B3) [NBP2-37566] - Western blot analysis using MCM2 mouse mAb against PC-12 (1), Cos7 (2), NIH/3T3 (3), HepG2 (4), HEK293 (5), K562 (6), Jurkat (7), Hela (8) and MCF-7 (9) cell lysate.

Immunohistochemistry: MCM2 Antibody (2B3) [NBP2-37566] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues using MCM2 mouse mAb with DAB staining.

Flow Cytometry: MCM2 Antibody (2B3) [NBP2-37566] - Flow cytometric analysis of Hela cells using MCM2 mouse mAb (blue) and negative control (red).

Western Blot: MCM2 Antibody (2B3) [NBP2-37566] - Western blot analysis using MCM2 mAb against human MCM2 (AA: 16-232) recombinant protein.(Expected MW is 50.4 kDa)

Immunohistochemistry: MCM2 Antibody (2B3) [NBP2-37566] - Immunohistochemical analysis of paraffin-embedded colon cancer tissues using MCM2 mouse mAb with DAB staining.

ELISA: MCM2 Antibody (2B3) [NBP2-37566] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu, Mu, Rt, Pm, Pm
• Applications: WB, ELISA, Flow, IHC
• Clone: 2B3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

MCM2 Antibody (2B3) Summary

• Description: Novus Biologicals Mouse MCM2 Antibody (2B3) (NBP2-37566) is a monoclonal antibody validated for use in IHC, WB, ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human MCM2 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MCM2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 125 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for MCM2 Antibody (2B3)

• BM28
• BM28MGC10606
• CCNL 1
• CCNL1
• cdc19
• CDCL1
• CDCL1mitotin
• D3S3194
• EC 3.6.4.12
• KIAA0030MITOTIN
• MCM2 minichromosome maintenance deficient 2, mitotin (S. cerevisiae)
• MCM2
• minichromosome maintenance complex component 2
• minichromosome maintenance deficient (S. cerevisiae) 2 (mitotin)
• minichromosome maintenance deficient 2 (mitotin)
• Mitotin

Background

MCM2 is encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MCM2