MCAF1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: MCAF1 Antibody [NBP2-97607] - Immunofluorescence staining of MCAF1 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human MCAF1 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.

• Reactivity: Hu
• Applications: ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MCAF1 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human MCAF1 fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATF7IP
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for MCAF1 Antibody - BSA Free

• activating transcription factor 7 interacting protein
• activating transcription factor 7-interacting protein 1
• ATF7-interacting protein
• ATFa-associated modulator
• ATF-interacting protein
• ATF-IP
• FLJ10139
• FLJ10688
• hAM
• MBD1-containing chromatin associated factor
• MCAF1AM
• MCAFMBD1-containing chromatin-associated factor 1
• MMCP 6
• MMCP6
• MMCP-6
• p621

Background

MBD1-containing chromatin-associated factor (MCAF) is a 1270-residue mediator in the DNA methylation cycle of a variety of genomic functions. This nuclear protein will bind to the C-terminal transcriptional repression domain of MBD1 to form a blocking complex. This molecule will then prevent transcription from methylated promoters in a histone deacetylation-independent action (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATF7IP