Western Blot: MCAF1 Antibody [NB100-438] - C11orf46 binds to the SETDB1 complex. Co-immunoprecipitation experiments using protein lysates from HEK293 cells overexpressing FLAG-tagged C11orf46, C11orf46delta, or ...read more
Immunohistochemistry: MCAF1 Antibody [NB100-438] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MCAF used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: MCAF1 Antibody [NB100-438] - Nuclear extract (20 ug) from HeLa cells. Antibody used at 0.2 and 0.1 ug/ml.
Biological Strategies: Western Blot: MCAF1 Antibody [NB100-438] - Detection of Human MCAF by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: ...read more
Immunohistochemistry-Paraffin: MCAF1 Antibody [NB100-438] - FFPE section of human small cell lung cancer. NB100-438 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit
activating transcription factor 7 interacting protein
activating transcription factor 7-interacting protein 1
MBD1-containing chromatin associated factor
MCAFMBD1-containing chromatin-associated factor 1
MBD1-containing chromatin-associated factor (MCAF) is a 1270-residue mediator in the DNA methylation cycle of a variety of genomic functions. This nuclear protein will bind to the C-terminal transcriptional repression domain of MBD1 to form a blocking complex. This molecule will then prevent transcription from methylated promoters in a histone deacetylation-independent action (1).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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