MCAF1 Antibody - BSA Free

Western Blot: MCAF1 Antibody [NB100-438] - C11orf46 binds to the SETDB1 complex. Co-immunoprecipitation experiments using protein lysates from HEK293 cells overexpressing FLAG-tagged C11orf46, C11orf46delta, or C11orf46R236H showed that absence of CRD domain and R236H mutation is associated with weakened or non-detectable C11orf46-SETDB1 binding and partial loss of binding to other components. R236H mutation does not fully disrupt C11orf46-KAP1 binding. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-12013-y), licensed under a CC-BY license.

Immunohistochemistry: MCAF1 Antibody [NB100-438] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MCAF used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Western Blot: MCAF1 Antibody [NB100-438] - Nuclear extract (20 ug) from HeLa cells. Antibody used at 0.2 and 0.1 ug/ml.

Western Blot: MCAF1 Antibody [NB100-438] - Detection of Human MCAF by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCAF antibody NB100-438 used for WB at 0.05 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry-Paraffin: MCAF1 Antibody [NB100-438] - FFPE section of human small cell lung cancer. NB100-438 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit

• Reactivity: Hu
• Applications: WB, IHC, WB, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

MCAF1 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide that maps to a region between residues 25 and 100 of human MBD1-containing Chromatin Associated Factor using the numbering given in entry AAQ92978.1 (GeneID 55729)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATF7IP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoblotting
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Knockdown Validated
  • Western Blot 1:5000-1:20000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Use in immunoblotting reported in scientific literature (PMID:31511512).
• Control:

  HeLa Nuclear Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MCAF1 Antibody - BSA Free

• activating transcription factor 7 interacting protein
• activating transcription factor 7-interacting protein 1
• ATF7-interacting protein
• ATFa-associated modulator
• ATF-interacting protein
• ATF-IP
• FLJ10139
• FLJ10688
• hAM
• MBD1-containing chromatin associated factor
• MCAF1AM
• MCAFMBD1-containing chromatin-associated factor 1
• MMCP 6
• MMCP6
• MMCP-6
• p621

Background

MBD1-containing chromatin-associated factor (MCAF) is a 1270-residue mediator in the DNA methylation cycle of a variety of genomic functions. This nuclear protein will bind to the C-terminal transcriptional repression domain of MBD1 to form a blocking complex. This molecule will then prevent transcription from methylated promoters in a histone deacetylation-independent action (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MCAF1 Antibody (NB100-438)(1)

Publication using NB100-438

• Peter CJ, Saito A, Hasegawa Y et al. In vivo epigenetic editing of Sema6a promoter reverses transcallosal dysconnectivity caused by C11orf46/Arl14ep risk gene Nat Commun 2019-09-11 [PMID: 31511512] (KD, IB, Human)

Bioinformatics

• Gene Symbol: ATF7IP
• Entrez:
  • 55729(Human)
• Uniprot:
  • Q6VMQ6(Human)