MBL-2 Antibody (14D12)


Immunohistochemistry-Frozen: Antibody (mAb 14D12) - MBL-C (clone 14D12) deposition in developing murine atherosclerotic lesions following 10 weeks of high fat feeding. MBL-C was detected in and around invading ...read more

Product Details

Reactivity MuSpecies Glossary
Applications WB, IA, ICC/IF, IHC, IHC-Fr
0.1 mg/ml

Order Details

MBL-2 Antibody (14D12) Summary

Mouse MBL-C
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
0.2 um filtered solution in PBS and 0.1% BSA
0.02% Sodium Azide
0.1 mg/ml
Protein A or G purified


  • Western Blot 1:100-1:2000
  • Immunoassay 1 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Application Notes
For IHC-F: Cryostat sections (7 um) of mouse tissue were acetone fixed and stained o/n at 4 degrees Celsius in TBS/2% goat serum. (Matthijsen, R et al). Immunoassay: Biotinylated 14D12 was used as detector in ELISA in TBS/Tween-20/Ca (see Liu, H et al). For IF: Hyphae of C. albicans were fixed on poly-l-lysin coated slides with ice-cold aceton. Slides were incubated for 1h at RT with 14D12 (see Held, K et al). For WB: On a 12% non-reducing SDS-PAGE a prominent band of ca. 50 kDa is detected. Under reducing conditions a 26 kDa band is detected (see Phaneuf, L et al).
Read Publications using NBP1-60128.

Reactivity Notes

Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.


Positive control: Mouse Serum and kidney tissue

Alternate Names for MBL-2 Antibody (14D12)

  • L-MBP
  • mannose-binding protein C
  • L-MBP
  • mannose-binding lectin (protein C) 2
  • MBL
  • MBL-C
  • MBP-C


Mannose binding lectin (MBL), also called mannose- or mannan-binding protein (MBP), is a member of the group of collectins. MBL is an important pattern-recognition receptor in the innate immune system. The protein mediates innate immune responses, such as activation of the complement lectin pathway and phagocytosis, to help fight infections. MBL is an oligomeric lectin that recognizes carbohydrates as mannose and N-acetylglucosamine on pathogens. MBL contains a cysteine rich, a collagen like and a carbohydrate recognition domain. Binding of MBL leads to the activation of MBL-associated serine proteases (MASP's). Activated MASP-2 cleaves C4 and C2 in a similar way as C1s do for the classical pathway (CP) leading to the formation of C4b2a, cleavage of the classical pathway convertase C3, and eventually complement activation up to the formation of the membrane attack complex. MBL is able to activate the complement pathway independent of the classical and alternative complement activation pathways. MBL is predominantly synthesized by hepatocytes and has been isolated from the liver or serum of several vertebrate species. Only one form of human MBL has been characterized, while two forms are found in rhesus monkeys, rabbits, rats and mice. The mouse forms are known as MBL-A and MBL-C. The MBL-C concentrations in serum are about 6-fold compared to that of MBL-A. MBL-A, but not MBL-C, was found to be an acute phase protein in casein and LPS-injection models. MBL-C exists in higher oligomeric forms than MBL-A. The monoclonal antibody 14D12 is a calcium-dependent antibody.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: WB, ICC/IF
Species: Mu
Applications: WB, Flow, IA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Species: Hu
Applications: ICC/IF, IHC-Fr, IHC-P
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt, Po, Bv, Ch, GP, Ma, Rb, Sh
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, RI
Species: Hu
Applications: WB, IHC, IHC-P, IF
Species: Hu
Applications: WB
Species: Hu
Applications: WB, ICC/IF, IHC
Species: Hu
Applications: IHC, IHC-Fr, IHC-P
Species: Hu
Applications: WB
Species: Hu, Mu, Rt
Applications: WB
Species: Hu, Mu, Rt, Ca
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for MBL-2 Antibody (NBP1-60128)(5)

Reviews for MBL-2 Antibody (NBP1-60128) (0)

There are no reviews for MBL-2 Antibody (NBP1-60128). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MBL-2 Antibody (NBP1-60128) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Additional MBL-2 Antibody (14D12) Products

MBL-2 NBP1-60128

Related Products by Gene

Bioinformatics Tool for MBL-2 Antibody (NBP1-60128)

Discover related pathways, diseases and genes to MBL-2 Antibody (NBP1-60128). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Blogs on MBL-2

There are no specific blogs for MBL-2, but you can read our latest blog posts.

Contact Information

Product PDFs


Gene Symbol Mbl2

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBP1-60128 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought