MBL-2 Antibody (14D12)

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Immunohistochemistry-Frozen: Antibody (mAb 14D12) - MBL-C (clone 14D12) deposition in developing murine atherosclerotic lesions following 10 weeks of high fat feeding. MBL-C was detected in and around invading ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IA, ICC/IF, IHC, IHC-Fr
Clone
14D12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.1 mg/ml

Order Details

MBL-2 Antibody (14D12) Summary

Immunogen
Mouse MBL-C
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
Mbl2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunoassay 1 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Application Notes
For IHC-F: Cryostat sections (7 um) of mouse tissue were acetone fixed and stained o/n at 4 degrees Celsius in TBS/2% goat serum. (Matthijsen, R et al). Immunoassay: Biotinylated 14D12 was used as detector in ELISA in TBS/Tween-20/Ca (see Liu, H et al). For IF: Hyphae of C. albicans were fixed on poly-l-lysin coated slides with ice-cold aceton. Slides were incubated for 1h at RT with 14D12 (see Held, K et al). For WB: On a 12% non-reducing SDS-PAGE a prominent band of ca. 50 kDa is detected. Under reducing conditions a 26 kDa band is detected (see Phaneuf, L et al).
Publications
Read Publications using NBP1-60128.

Reactivity Notes

Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.2 um filtered solution in PBS and 0.1% BSA
Preservative
0.02% Sodium Azide
Concentration
0.1 mg/ml
Purity
Protein A or G purified

Notes

Positive Control: Mouse Serum and kidney tissue

Alternate Names for MBL-2 Antibody (14D12)

  • L-MBP
  • mannose-binding protein C
  • L-MBP
  • mannose-binding lectin (protein C) 2
  • MBL
  • MBL-2
  • MBL-C
  • MBP-C

Background

Mannose binding lectin (MBL), also called mannose- or mannan-binding protein (MBP), is a member of the group of collectins. MBL is an important pattern-recognition receptor in the innate immune system. The protein mediates innate immune responses, such as activation of the complement lectin pathway and phagocytosis, to help fight infections. MBL is an oligomeric lectin that recognizes carbohydrates as mannose and N-acetylglucosamine on pathogens. MBL contains a cysteine rich, a collagen like and a carbohydrate recognition domain. Binding of MBL leads to the activation of MBL-associated serine proteases (MASP's). Activated MASP-2 cleaves C4 and C2 in a similar way as C1s do for the classical pathway (CP) leading to the formation of C4b2a, cleavage of the classical pathway convertase C3, and eventually complement activation up to the formation of the membrane attack complex. MBL is able to activate the complement pathway independent of the classical and alternative complement activation pathways. MBL is predominantly synthesized by hepatocytes and has been isolated from the liver or serum of several vertebrate species. Only one form of human MBL has been characterized, while two forms are found in rhesus monkeys, rabbits, rats and mice. The mouse forms are known as MBL-A and MBL-C. The MBL-C concentrations in serum are about 6-fold compared to that of MBL-A. MBL-A, but not MBL-C, was found to be an acute phase protein in casein and LPS-injection models. MBL-C exists in higher oligomeric forms than MBL-A. The monoclonal antibody 14D12 is a calcium-dependent antibody.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MBL-2 Antibody (NBP1-60128)(5)

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol Mbl2
Entrez