Maxi Potassium channel beta Antibody

Western Blot: Maxi Potassium channel beta Antibody [NB300-535] - Analysis of Maxi K+ Beta (KCNMB1) was performed by loading 20ug of Movas whole cell lysates with and without Maxi K+ Beta (KCNMB1) siRNA and 10ul prestained protein ladder per well onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% Milk/TBST for at least 1 hour at room temperature. Maxi K+ Beta was detected using a Maxi K+ Beta rabbit polyclonal antibody at a concentration of 1ug/ml in blocking buffer overnight at 4 degrees C on a rocking platform, followed by a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5,000 for at least 1 hour at room temperature. Chemiluminescent detection was performed.

Immunocytochemistry/Immunofluorescence: Maxi Potassium channel beta Antibody [NB300-535] - analysis of Maxi K+ Beta (green) in Movas cells transfected with (+) and without (-) Maxi K+ Beta siRNA. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 10 minutes, and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were stained with a p38 polyclonal antibody at a dilution of 1:100 in staining buffer for 1 hour at room temperature, and then incubated with a Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 conjugate at a dilution of 1:1000 for 1 hour at room temperature (green). F-actin (red) was stained by Dylight 554 Phalloidin and Nuclei (blue) were stained with Hoechst dye.

Western Blot: Maxi Potassium channel beta Antibody [NB300-535] - Analysis of Maxi K+ Beta was performed by loading 20ug of Movas whole cell lysates and 10ul prestained protein ladder per well onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane u and blocked with 5% Milk/TBST for at least 1 hour at room temperature. Maxi K+ Beta was detected using a Maxi K+ rabbit polyclonal antibody at a concentration of 1ug/ml in blocking buffer overnight at 4 degrees C on a rocking platform, followed by a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5,000 for at least 1 hour at room temperature. Chemiluminescent detection was performed.

Immunocytochemistry/Immunofluorescence: Maxi Potassium channel beta Antibody [NB300-535] - Analysis of Maxi K+ Beta (green) in Movas cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 10 minutes, and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were stained with a Maxi K+ Beta polyclonal antibody at a dilution of 1:100 in staining buffer for 1 hour at room temperature, and then incubated with a Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 conjugate at a dilution of 1:1000 for 1 hour at room temperature (green). F-actin (red) was stained by Dylight 554 Phalloidin and Nuclei (blue) were stained with Hoechst dye.

• Reactivity: Hu, Mu, Rt, Po, Ca, Rb
• Applications: WB, Flow, ICC/IF, IHC, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Maxi Potassium channel beta Antibody Summary

• Immunogen: Synthetic Peptide: L(90) Y H T E D T R D Q N Q Q C(103)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KCNMB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:200
  • Immunohistochemistry 1:200 - 1:2000
  • Immunohistochemistry-Paraffin 1:200 - 1:2000
  • Knockdown Validated
  • Western Blot 2.0 ug/ml
• Application Notes: Use in Flow Cytometry reported in scientific literature (PMID:32265729).
• Control Peptide:

  Maxi Potassium channel beta Protein (NB300-535PEP)

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 15613616). Caniine (PMID: 20576683). Porcine (PMID: 19749164).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Maxi Potassium channel beta Antibody

• BK channel beta subunit
• BK channel subunit beta-1
• BKbeta
• BKbeta1
• Calcium-activated potassium channel subunit beta
• calcium-activated potassium channel subunit beta-1
• Calcium-activated potassium channel, subfamily M subunit beta-1
• Charybdotoxin receptor subunit beta-1
• Hbeta1
• hslo-beta
• K(VCA)beta
• K(VCA)beta-1
• KCNMB1
• large conductance Ca2+-activated K+ channel beta 1 subunit
• Maxi K channel beta subunit
• Maxi K channel subunit beta-1
• Maxi Potassium channel beta
• potassium large conductance calcium-activated channel, subfamily M, beta member1
• Slo-beta
• Slo-beta-1

Background

Potassium channels are a group of ubiquitously expressed proteins that serve numerous functions in excitable and non-excitable cells. One class of integral membrane potassium channels is the large conductance, calcium-activated potassium channel (Maxi K+). Maxi K+ differs from most other potassium channels in that its activation is controlled by both increases in intracellular calcium and by membrane depolarization. Maxi K+ dual activation is possible because of its structure. The core of the channel, which is similar to other potassium channels, is a Maxi K+ alpha homotetramer that contains both a voltage sensor and an intracellular calcium binding domain. In vascular smooth muscle, an auxiliary beta-subunit is found in a 1:1 stoichiometry. The beta-subunit exhibits its effect on the Maxi K+ channel by effectively decreasing by 5- to 10- fold the concentration of calcium required to keep the pore open. Maxi K+ beta is the target for possible therapeutics because of its role in blood flow and blood pressure regulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Maxi Potassium channel beta Antibody (NB300-535)(7)

Publications using NB300-535

• Feher A, Petho Z, Szanto TG et al. Mapping the functional expression of auxiliary subunits of KCa1.1 in glioblastoma Scientific reports 2022-12-20 [PMID: 36539587] (WB, Human)
• Wang LF, Ling DY, Huang MX Et al. Influence of atherosclerosis on the molecular expression of the TRPC1/BK signal complex in the aortic smooth muscles of mice Experimental and therapeutic medicine 2022-01-01 [PMID: 34815756] (WB, IHC-P, Mouse)
• Echeverry S, Grismaldo A, SAnchez C et al. Activation of BK Channel Contributes to PL-Induced Mesenchymal Stem Cell Migration Front Physiol 2020-03-24 [PMID: 32265729] (WB, FLOW, Rat)
• Borbouse L, Dick GM, Asano S, Bender SB, Dincer UD, Payne GA, Neeb ZP, Bratz IN, Sturek M, Tune JD. Impaired function of coronary BK(Ca) channels in metabolic syndrome. Am J Physiol Heart Circ Physiol 297(5):H1629-37. 2009-11-01 [PMID: 19749164]
• Martin G, O'Connell RJ, Pietrzykowski AZ et al. Interleukin-4 activates large-conductance, calcium-activated potassium (BKCa) channels in human airway smooth muscle cells. Exp Physiol. 2008-07-01 [PMID: 18403443]
• Alioua, A, R Lu et al. Slo1 Caveolin-binding Motif, a Mechanism of Caveolin-1-Slo1 Interaction Regulating Slo1 Surface Expression. J Biol Chem 283: 4808-4817. 2008-01-01 [PMID: 18079116] (ICC/IF)
• Martin G, Puig S, Pietrzykowski A, Zadek P, Emery P, Treistman S. Somatic localization of a specific large-conductance calcium-activated potassium channel subtype controls compartmentalized ethanol sensitivity in the nucleus accumbens. J Neurosci 21;24(29):6563-72. 2004-07-01 [PMID: 15269268]

Bioinformatics

• Gene Symbol: KCNMB1
• Entrez:
  • 3779(Human)
  • 16533(Mouse)
  • 29747(Rat)
• Uniprot:
  • Q16558(Human)