MATK Overexpression Lysate

Images

 
Western Blot: MATK Overexpression Lysate (Adult Normal) [NBL1-12913] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MATK.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

MATK Overexpression Lysate Summary

Description
Transient overexpression lysate of megakaryocyte-associated tyrosine kinase (MATK), transcript variant 3 The lysate was created in HEK293T cells, using Plasmid ID RC200454 and based on accession number NM_139354. The protein contains a C-MYC/DDK Tag.
Gene
MATK

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
51.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for MATK Overexpression Lysate

  • CHK
  • Csk-type protein tyrosine kinase
  • CTKEC 2.7.10.2
  • DKFZp434N1212
  • EC 2.7.10
  • Hematopoietic consensus tyrosine-lacking kinase
  • HHYLTK
  • hydroxyaryl-protein kinase
  • HYL tyrosine kinase
  • HYLCsk-homologous kinase
  • HYLTK
  • leukocyte carboxyl-terminal src kinase related
  • Lsk
  • megakaryocyte-associated tyrosine kinase
  • megakaryocyte-associated tyrosine-protein kinase
  • MGC1708
  • MGC2101
  • Protein kinase HYL
  • tyrosine kinase MATK
  • Tyrosine-protein kinase CTK
  • tyrosylprotein kinase

Background

The protein encoded by this gene has amino acid sequence similarity to Csk tyrosine kinase and has the structural features of the CSK subfamily: SRC homology SH2 and SH3 domains, a catalytic domain, a unique N terminus, lack of myristylation signals, lack of a negative regulatory phosphorylation site, and lack of an autophosphorylation site. This protein is thought to play a significant role in the signal transduction of hematopoietic cells. It is able to phosphorylate and inactivate Src family kinases, and may play an inhibitory role in the control of T-cell proliferation. This protein might be involved in signaling in some cases of breast cancer. Three alternatively spliced transcript variants that encode different isoforms have been described for this gene. [provided by RefSeq]. Transcript Variant: This variant (3) lacks an exon in the coding region, and utilizes a downstream in-frame start codon, compared to variant 1. Isoform c has a shorter N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF2685
Species: Hu
Applications: IHC, Simple Western, WB
NBP1-85951
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NB100-464
Species: Hu, Mu
Applications: IHC, IHC-P, IP, WB
AF1356
Species: Hu, Mu
Applications: CyTOF-ready, Flow, IHC, Simple Western, WB
NB100-56708
Species: Ch, ChHa, Hu, Ma, Mu, Po, Rt
Applications: CyTOF-ready, EM, Flow, HEStain, IB, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, KD, KO, Simple Western, WB
NB100-104
Species: Ca, Hu, Ma, Mu, Rt
Applications: ELISA, Flow, IB, ICC/IF, IHC, IHC-P, IP, WB
NB200-103
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
255-SC/CF
Species: Hu
Applications: BA
1129-ER
Species: Hu
Applications: BA
AF3206
Species: Hu, Mu, Rt
Applications: ICC, Simple Western, WB
NBP1-80025
Species: Hu
Applications: IHC, IHC-P, WB
H00002523-P01
Species: Hu
Applications: ELISA, AP, PA, WB
NBP2-22203
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
NBP1-90816
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KD, WB
NBP2-03417
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
NBP2-67627
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
MAB5285
Species: Hu
Applications: Simple Western, WB

Publications for MATK Lysate (NBL1-12913) (0)

There are no publications for MATK Lysate (NBL1-12913).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for MATK Lysate (NBL1-12913) (0)

There are no reviews for MATK Lysate (NBL1-12913). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MATK Lysate (NBL1-12913) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional MATK Products

Bioinformatics Tool for MATK Lysate (NBL1-12913)

Discover related pathways, diseases and genes to MATK Lysate (NBL1-12913). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for MATK Lysate (NBL1-12913)

Discover more about diseases related to MATK Lysate (NBL1-12913).
 

Pathways for MATK Lysate (NBL1-12913)

View related products by pathway.

PTMs for MATK Lysate (NBL1-12913)

Learn more about PTMs related to MATK Lysate (NBL1-12913).
 

Research Areas for MATK Lysate (NBL1-12913)

Find related products by research area.

Blogs on MATK

There are no specific blogs for MATK, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our MATK Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol MATK