MATK Overexpression Lysate

Western Blot: MATK Overexpression Lysate (Adult Normal) [NBL1-12913] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MATK.

• Reactivity: Hu
• Applications: WB

MATK Overexpression Lysate Summary

• Description: Transient overexpression lysate of megakaryocyte-associated tyrosine kinase (MATK), transcript variant 3 The lysate was created in HEK293T cells, using Plasmid ID RC200454 and based on accession number NM_139354. The protein contains a C-MYC/DDK Tag.
• Gene: MATK

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
• Theoretical MW: 51.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for MATK Overexpression Lysate

• CHK
• Csk-type protein tyrosine kinase
• CTKEC 2.7.10.2
• DKFZp434N1212
• EC 2.7.10
• Hematopoietic consensus tyrosine-lacking kinase
• HHYLTK
• hydroxyaryl-protein kinase
• HYL tyrosine kinase
• HYLCsk-homologous kinase
• HYLTK
• leukocyte carboxyl-terminal src kinase related
• Lsk
• megakaryocyte-associated tyrosine kinase
• megakaryocyte-associated tyrosine-protein kinase
• MGC1708
• MGC2101
• Protein kinase HYL
• tyrosine kinase MATK
• Tyrosine-protein kinase CTK
• tyrosylprotein kinase

Background

The protein encoded by this gene has amino acid sequence similarity to Csk tyrosine kinase and has the structural features of the CSK subfamily: SRC homology SH2 and SH3 domains, a catalytic domain, a unique N terminus, lack of myristylation signals, lack of a negative regulatory phosphorylation site, and lack of an autophosphorylation site. This protein is thought to play a significant role in the signal transduction of hematopoietic cells. It is able to phosphorylate and inactivate Src family kinases, and may play an inhibitory role in the control of T-cell proliferation. This protein might be involved in signaling in some cases of breast cancer. Three alternatively spliced transcript variants that encode different isoforms have been described for this gene. [provided by RefSeq]. Transcript Variant: This variant (3) lacks an exon in the coding region, and utilizes a downstream in-frame start codon, compared to variant 1. Isoform c has a shorter N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: MATK