Mast Cell Protease-1/Mcpt1 Antibody (285008) [Unconjugated]

Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse/Rat Mast Cell Protease-1/Mcpt1 Monoclonal Antibody (Catalog # MAB5146) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Mast Cell Protease-1/Mcpt1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of mouse small intestine tissue, loaded at 0.2 mg/mL. A specific band was detected for Mast Cell Protease‑1/Mcpt1 at approximately 34 kDa (as indicated) using 50 µg/mL of Rat Anti-Mouse/Rat Mast Cell Protease‑1/Mcpt1 Monoclonal Antibody (Catalog # MAB5146) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Mu
• Applications: WB, Simple Western
• Clone: 285008
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Mast Cell Protease-1/Mcpt1 Antibody (285008) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Mast Cell Protease‑1/Mcpt1
  Ile21-Lys246
  Accession # P11034
• Specificity: Detects mouse Mast Cell Protease-1/Mcpt1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) MCP-6, -7, or -11 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: Mcpt1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Simple Western 50 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mast Cell Protease-1/Mcpt1 Antibody (285008) [Unconjugated]

• AV080368
• Mast Cell Protease1
• Mast Cell Protease-1
• Mcp1
• Mcp-1
• MMPC-1

Background

Mast cell protease-1 (MCPT-1), also known as beta -chymase, is a member of the Chymase family of chymotrypsin-like serine proteases (1). mMCPT-1 is a mast cell protease predominantly expressed in intestinal mucosal mast cells where it promotes mucosal permeability in intestinal allergic hypersensitivity reactions (2). Its activation is completed by the removal of a two residue N-terminal propeptide by a dipeptidyl peptidase (Cathepsin C) (3). Like human alpha -Chymase, MCPT-1 is capable of the conversion of angiotensin I to angiotensin II, which plays a key role in the regulation of arterial pressure (4). Studies have shown that specific chymase inhibitors are able to diminish the development of abdominal aortic aneurysm and reduce the adhesion formation after cardiac surgery in hamsters (5, 6). Therefore, the development of specific inhibitors of chymase activity has been a pharmacologic strategy to develop therapeutic agents.

1. Caughey, G.H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.), p. 1531 Academic Press, San Diego.
2. Wastling, J.M. et al. (1998) Am. J. Pathol. 153:491.
3. Murakami, M. et al. (1995) J. Biol. Chem. 270:2218.
4. Saito, K. et al. (2003) Biochem. Biophys. Res. Commun. 302:773.
5. Tsunemi, K. et al. (2004) J. Pharmacol. Exp. Ther. 309:879.
6. Soga, Y. et al. (2004) J. Thorac. Cardiovasc. Surg. 127:72.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Mast Cell Protease-1/Mcpt1 Antibody (MAB5146)(2)

Publications using MAB5146

• Joris van der Veeken, Clarissa Campbell, Yuri Pritykin, Michail Schizas, Jacob Verter, Wei Hu et al. Genetic tracing reveals transcription factor Foxp3-dependent and Foxp3-independent functionality of peripherally induced Treg cells Immunity 2022-07-01 [PMID: 35700740]
• Dmitriy Sheyn, Doron Cohn Cohn Yakubovich, Ilan Kallai, Susan Su, Xiaoyu Da, Gadi Pelled et al. PTH Promotes Allograft Integration in a Calvarial Bone Defect Molecular Pharmaceutics 2013-12-02 [PMID: 24131143]

Bioinformatics

• Gene Symbol: Mcpt1
• Uniprot:
  • P11034()