MAS1L Antibody (15B8) - BSA Free

Western Blot: MAS1L Antibody (15B8) [NBP3-26148] - Positive Western Blot detected in: MCF-7 whole cell lysate, Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, PC-3 whole cell lysate, Jurkat whole cell lysate, Rat liver tissue. All lanes: MAS1L Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 43 kDa Observed band size: 36-50 kDa

Immunocytochemistry/Immunofluorescence: MAS1L Antibody (15B8) [NBP3-26148] - Staining of MCF-7 cell with MAS1L Antibody (15B8) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 595-conjugated Goat Anti-Rabbit IgG (H+L).

Immunocytochemistry/Immunofluorescence: MAS1L Antibody (15B8) [NBP3-26148] - Staining of Hela cell with MAS1L Antibody (15B8) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 595-conjugated Goat Anti-Rabbit IgG (H+L).

Flow Cytometry: MAS1L Antibody (15B8) [NBP3-26148] - Overlay Peak curve showing Hela cells stained with MAS1L Antibody (15B8) (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: 15B8
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MAS1L Antibody (15B8) - BSA Free Summary

• Description: Novus Biologicals Rabbit MAS1L Antibody (15B8) - BSA Free (NBP3-26148) is a recombinant monoclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from Human MAS1L [UniProt P35410]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MAS1L
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for MAS1L Antibody (15B8) - BSA Free

• DJ994E9.2
• MAS1 Oncogene-Like
• MAS1L
• MAS1-Like
• MAS-L
• MAS-R
• mas-related G protein-coupled MRG
• Mas-Related G-Protein Coupled Receptor MRG
• MRG
• MRGMGC119987

Background

MRG is an Orphan-A GPCR with an unknown ligand highly similar to other MRG receptors. It is most similar to the product of the MAS protooncogene, which controls cellular responses to angiotensin. MRG has been reported absent in the adrenal, liver, kidney, and vascular smooth muscle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAS1L