Mark3 Antibody (OTI2G8) [mFluor Violet 610 SE]

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: OTI2G8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: mFluor Violet 610 SE

Mark3 Antibody (OTI2G8) [mFluor Violet 610 SE] Summary

• Immunogen: Human recombinant protein fragment corresponding to amino acids 473-729 of human MARK3(NP_002367) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MARK3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Mark3 Antibody (OTI2G8) [mFluor Violet 610 SE]

• Cdc25C-associated protein kinase 1
• cTAK1
• C-TAK1
• CTAK1ELKL motif kinase 2
• EC 2.7.11
• EC 2.7.11.1
• EMK2
• EMK-2
• KP78
• MAP/microtubule affinity-regulating kinase 3
• PAR1A
• Protein kinase STK10
• Ser/Thr protein kinase PAR-1
• Serine/threonine-protein kinase p78

Background

MARK3 is a dual-specificity protein kinase that controls entry into mitosis by dephosphorylating Cdc2 on both threonine 14 and tyrosine 15. MARK3 is phosphorylated on serine 216 throughout interphase but not during mitosis. Serine 216 phosphorylation mediates the binding of 14-3-3 protein to MARK3, and MARK3/14-3-3 complexes are present throughout interphase but not during mitosis (1). Kinase suppressor of Ras (KSR) is a conserved component of the Ras pathway that interacts directly with MEK and MAPK. It has been shown that KSR1 translocates from the cytoplasm to the cell surface in response to growth factor treatment and that this process is regulated by MARK3 (2). MARK3 seems to be a positive regulator of the beta-catenin pathway and an inhibitor of the JNK pathway. These findings show that MARK3, a regulator of polarity, is also a modulator of Wnt-beta-catenin signalling, indicating a link between two important developmental pathways (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MARK3