MARK2 Antibody (JE40-97)

Western Blot: MARK2 Antibody (JE40-97) [NBP2-75566] - Analysis of MARK2 on MCF-7 (1) and SK-Br-3 (2) cell lysate using anti-MARK2 antibody at 1/2,000 dilution.

Immunohistochemistry-Paraffin: MARK2 Antibody (JE40-97) [NBP2-75566] - Analysis of paraffin-embedded human appendix tissue using anti-MARK2 antibody. Counter stained with hematoxylin.

Flow Cytometry: MARK2 Antibody (JE40-97) [NBP2-75566] - Analysis of MCF-7 cells with MARK2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Product Discontinued

MARK2 Antibody (JE40-97) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human MARK2 aa 661-789 / 789. (SwissProt: Q7KZI7 Human)
• Localization: Cell membrane. Cytoplasm. Cytoskeleton.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MARK2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 88 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for MARK2 Antibody (JE40-97)

• EC 2.7.11
• EC 2.7.11.1
• ELKL motif kinase 1
• ELKL motif kinase
• EMK-1
• EMK1PAR-1
• MAP/microtubule affinity-regulating kinase 2MGC99619
• PAR1 homolog
• Par1b
• Ser/Thr protein kinase PAR-1B
• serine/threonine protein kinase EMK
• serine/threonine-protein kinase MARK2

Background

EMK (ELKL Motif Kinase) is a small family of ser/thr protein kinases involved in the control of cell polarity, microtubule stability and cancer. Several cDNA clones have been isolated that encoded two isoforms of the human ser/thr protein kinase EMK1. These isoforms were characterized by the presence of a 162-bp alternative exon that gave rise to two forms, one containing the exon and the other one lacking it. Both forms were found to be coexpressed in a number of selected cell lines and tissue samples. The human EMK1 was shown to be encoded by a single mRNA ubiquitously expressed. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MARK2