MARCKS Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: MARCKS Antibody [NBP2-68861] - Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.
Immunocytochemistry/ Immunofluorescence: MARCKS Antibody [NBP2-68861] - Staining of human cell line U-251 MG shows localization to plasma membrane & endoplasmic reticulum.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

MARCKS Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: DPQEAKPQEAAVAPEKPPASDETKAAEEPSKVEEK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MARCKS
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:2500 - 1:5000
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
Application Notes
Recommended conditions for ICC/IF: Fixation/Permeabilization: PFA/Triton X-100, Recommended conditions for IHC: Retrieval method: HIER pH6

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for MARCKS Antibody

  • 80K-L protein
  • 80K-L
  • FLJ14368
  • MACS
  • MACSmyristoylated alanine-rich C-kinase substrate
  • MARCKS
  • MRACKS
  • myristoylated alanine-rich protein kinase C substrate (MARCKS, 80K-L)
  • myristoylated alanine-rich protein kinase C substrate
  • phosphomyristin
  • PKCSLFLJ90045
  • PRKCSL
  • Protein kinase C substrate, 80 kDa protein, light chain

Background

Myristolyated alanine rich C-kinase substrate, hence MARCKS, was originally discovered as a major substrate for protein kinase C in the brain and other tissues, and was originally isolated from human epithelial cells. MARCKS is a major protein of brain, and is concentrated in synapses of neurons. It appears to function in synaptic vesicle cycling and has been shown to bind both actin and calmodulin in vitro. MARCKS belongs to a family of proteins with similar actin and calmodulin binding properties. Deletion of the MARCKS gene in mice results in embryonic brain defects and death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MARCKS