MARCH5 Antibody

Western Blot: MARCH5 Antibody [NBP2-21583] - Sample (20 ug of whole cell lysate) A: HeLa B: HeLa membrane, 12% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: MARCH5 Antibody [NBP2-21583] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using 39145 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.

Product Discontinued

MARCH5 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human MARCH5. The exact sequence is proprietary.
• Localization: Mitochondrion outer membrane; Multi-pass membrane protein
• Predicted Species: Mouse (100%), Rat (100%), Chicken (99%), Xenopus (98%), Zebrafish (99%), Rhesus Macaque (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MARCH5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry
  • Western Blot 1:500-1:3000
• Application Notes: Use in IHC reported in scientific literature (PMID: 30636894).
• Theoretical MW: 31 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (98%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for MARCH5 Antibody

• E3 ubiquitin-protein ligase MARCH5
• EC 6.3.2
• EC 6.3.2.-
• FLJ20445
• MARCH-VRNF153
• membrane-associated ring finger (C3HC4) 5
• Membrane-associated RING finger protein 5
• Membrane-associated RING-CH protein V
• Mitochondrial ubiquitin ligase
• RING finger protein 153MITOL

Background

MARCH5 is a ubiquitin ligase of the mitochondrial outer membrane that plays a role in the control of mitochondrial morphology by regulating mitofusin-2 (MFN2; MIM 608507) and DRP1 (DNM1L; MIM 603850) (Nakamura et al., 2006 [PubMed 16936636]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for MARCH5 Antibody (NBP2-21583)(2)

Publications using NBP2-21583

• Lei W, Li J, Li C et al. MARCH5 restores endothelial cell function against ischaemic/hypoxia injury via Akt/eNOS pathway Journal of cellular and molecular medicine 2021-02-21 [PMID: 33611830]
• Tang H, Peng S, Dong Y et al. MARCH5 overexpression contributes to tumor growth and metastasis and associates with poor survival in breast cancer. Cancer Manag Res 1905-07-11 [PMID: 30636894] (IF/IHC, WB, Human)

Bioinformatics

• Gene Symbol: MARCH5
• Uniprot:
  • Q9NX47(Human)