MAP1B Antibody - BSA Free

Immunoprecipitation: MAP1B Antibody [NB100-68256] - Detection of human MAP1B by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MAP1B antibody NB100-68256 (lot NB100-68256-2) used for IP at 6 ug per reaction. MAP1B was also immunoprecipitated by a previous lot of this antibody (lot NB100-68256-1) and another rabbit anti-MAP1B antibody. For blotting immunoprecipitated MAP1B, NB100-68256 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds

• Reactivity: Hu, Mu, Rt
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

MAP1B Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit MAP1B Antibody - BSA Free (NB100-68256) is a polyclonal antibody validated for use in WB and IP. Anti-MAP1B Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 900 and 950 of human microtubule-associated protein 1B using the numbering given in entry NP_005900.2 (GeneID 4131).
• Marker: Neuronal Marker
• Predicted Species: Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAP1B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot
• Application Notes: WB reported in scientific literature (PMID:24664738).

Reactivity Notes

Mouse reactivity reported in (PMID:24664738).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MAP1B Antibody - BSA Free

• DKFZp686E1099
• DKFZp686F1345
• FLJ38954
• FUTSCH
• MAP-1B
• MAP5
• microtubule-associated protein 1B

Background

This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The product of this gene is a precursor polypeptide that presumably undergoes proteolytic processing to generate the final MAP1B heavy chain and LC1 light chain. Gene knockout studies of the mouse microtubule-associated protein 1B gene suggested an important role in development and function of the nervous system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MAP1B Antibody (NB100-68256)(3)

Publications using NB100-68256

• Iketani M, Hatomi M, Fujita Y, Watanabe N et Al. Inhalation of hydrogen gas mitigates sevoflurane-induced neuronal apoptosis in the neonatal cortex and is associated with changes in protein phosphorylation J Neurochem 2024-06-07 [PMID: 38849977]
• Ebke LA, Sinha S, Pauer GJT, Hagstrom SA. Photoreceptor Compartment-Specific TULP1 Interactomes International Journal of Molecular Sciences 2021-07-28 [PMID: 34360830] (Immunoprecipitation, Western Blot, Mouse)
• Grossman GH, Beight CD, Ebke LA et al. Interaction of Tubby-Like Protein-1 (Tulp1) and Microtubule-Associated Protein (MAP) 1A and MAP1B in the Mouse Retina. Adv. Exp. Med. Biol. 2014-03-25 [PMID: 24664738] (WB, IP, Mouse)

Bioinformatics

• Gene Symbol: MAP1B
• Entrez:
  • 4131(Human)
• Uniprot:
  • P46821(Human)