MAK Antibody (4E9) - Azide and BSA Free

Western Blot: MAK Antibody (4E9) [H00004117-M02] - Analysis of MAK expression in transfected 293T cell line by MAK monoclonal antibody (M02), clone 4E9.Lane 1: MAK transfected lysate(52.6 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: MAK Antibody (4E9) [H00004117-M02] - Analysis of monoclonal antibody to MAK on HeLa cell. Antibody concentration 10 ug/ml.

Western Blot: MAK Antibody (4E9) [H00004117-M02] - MAK monoclonal antibody (M02), clone 4E9 Analysis of MAK expression in Hela S3 NE.

Sandwich ELISA: MAK Antibody (4E9) [H00004117-M02] - Detection limit for recombinant GST tagged MAK is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 4E9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MAK Antibody (4E9) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: MAK (AAH39825, 358 a.a. ~ 457 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PPKQQSQEKPPQTLFPSIVKNMPTKPNGTLSHKSGRRRWGQTIFKSGDSWEELEDYDFGASHSKKPSMGVFKEKRKKDSPFRQQVKMAVISLSAHQFPTL
• Specificity: MAK (4E9)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAK
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MAK Antibody (4E9) - Azide and BSA Free

• dJ417M14.2
• EC 2.7.11
• EC 2.7.11.22
• male germ cell-associated kinaseserine/threonine protein kinase MAK
• serine/threonine-protein kinase MAK

Background

The product of this gene is a serine/threonine protein kinase related to kinases involved in cell cycle regulation. It is expressed almost exclusively in the testis, primarily in germ cells. Studies of the mouse and rat homologs have localized the kinase to the chromosomes during meiosis in spermatogenesis, specifically to the synaptonemal complex that exists while homologous chromosomes are paired. There is, however, a study of the mouse homolog that has identified high levels of expression in developing sensory epithelia so its function may be more generalized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAK
• Entrez:
  • 4117(Human)
• OMIM:
  • 154235(Human)
• Uniprot:
  • AAH39825(Human)