MAGI3 Antibody

Images

 
Western Blot: MAGI3 Antibody [NBP2-17210] - Intronic pPA of MAGI3 occurs following the gene's large internal exon. Full length MAGI3 and truncated MAGI3pPA proteins are detected by immunoblotting. Immunoblot of ...read more
Western Blot: MAGI3 Antibody [NBP2-17210] -Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MAGI3 antibody [N1N2], N-term diluted at 1:1000. The HRP-conjugated ...read more
Expression of truncated MAGI3 leads to increased YAP activation and YAP-dependent transformation. (E) Immunoblots of V5-tagged MAGI3pPA, MAGI3deltaC-GFP, Ser127-phosphorylated YAP, total YAP and beta-actin levels in ...read more
Intronic pPA of MAGI3 occurs following the gene's large internal exon. (D) Full length MAGI3 and truncated MAGI3pPA proteins are detected by immunoblotting. Immunoblot of beta-actin is included to show loading, ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, ChSpecies Glossary
Applications WB, ICC/IF, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

MAGI3 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human MAGI3. The exact sequence is proprietary.
Predicted Species
Mouse (96%), Rat (95%), Chicken (93%), Bovine (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAGI3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence -Reported in scientific literature (PMID: 27205883)
  • Immunoprecipitation -Reported in scientific literature (PMID: 27205883)
  • Western Blot 1:500-1:3000
Theoretical MW
166 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-17210 in the following applications:

  • 1 publication
  • IP
    1 publication
  • WB
    3 publications

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS pH7, 1% BSA, 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for MAGI3 Antibody

  • dJ730K3.2
  • MAGI-3
  • membrane associated guanylate kinase, WW and PDZ domain containing 3
  • membrane-associated guanylate kinase inverted 3
  • membrane-associated guanylate kinase-related 3
  • WW and PDZ domain-containing protein 3

Background

Acts as a scaffolding protein at cell-cell junctions, thereby regulating various cellular and signaling processes. Cooperates with PTEN to modulate the kinase activity of AKT1. Its interaction with PTPRB and tyrosine phosphorylated proteins suggests that it may link receptor tyrosine phosphatase with its substrates at the plasma membrane. In polarized epithelial cells, involved in efficient trafficking of TGFA to the cell surface. Regulates the ability of LPAR2 to activate ERK and RhoA pathways. Regulates the JNK signaling cascade via its interaction with FZD4 and VANGL2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for MAGI3 Antibody (NBP2-17210)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol MAGI3
Uniprot