MAGEA3 Antibody (OTI1G9)

Western Blot: MAGEA3 Antibody (1G9) [NBP2-45719] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAGEA3.

Immunohistochemistry: MAGEA3 Antibody (1G9) [NBP2-45719] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Western Blot: MAGEA3 Antibody (1G9) [NBP2-45719] - Analysis of extracts (10ug) from MCF7 cell line.

Immunohistochemistry: MAGEA3 Antibody (1G9) [NBP2-45719] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

• Reactivity: Hu
• Applications: WB, IHC
• Clone: OTI1G9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

MAGEA3 Antibody (OTI1G9) Summary

• Immunogen: Full length human recombinant protein of human MAGEA3 (NP_005353) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAGEA3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Theoretical MW: 34.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MAGEA3 Antibody (OTI1G9)

• Antigen MZ2-D
• Cancer/testis antigen 1.3
• CT1.3 MAGE-3 antigen
• CT1.3
• HIP8
• MAGE3 MAGEA6
• MAGE3
• MAGEA3
• melanoma antigen family A, 3
• melanoma-associated antigen 3
• member 3
• MGC14613

Background

MAGEA3, also known as Melanoma-associated antigen 3, is a 34.7 kDa, 314 amino acid protein that is involved in increasing ubiquitin ligase activity as a member of the MAGEA family. Current research is being conducted to study the effects of the protein on diseases and disorders such as bile duct cancer, dyskeratosis, ocular melanoma, germ cell tumors, multiple types of carcinomas, avian influenza, testicular cancer, lung cancer, and lymphoma. The protein interacts with other proteins such as TRIM28, UBC, HTT, and SUMO2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAGEA3