MAdCAM-1 Antibody (OTI1A5)

Western Blot: MAdCAM-1 Antibody (OTI1A5) [NBP2-45706] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MADCAM-1.

Immunofluorescence: MAdCAM-1 Antibody (OTI1A5) [NBP2-45706] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY MADCAM1.

• Reactivity: Hu, Po
• Applications: WB, ICC/IF
• Clone: OTI1A5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

MAdCAM-1 Antibody (OTI1A5) Summary

• Description: Novus Biologicals Mouse MAdCAM-1 Antibody (OTI1A5) (NBP2-45706) is a monoclonal antibody validated for use in WB and ICC/IF. Anti-MAdCAM-1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human MADCAM1(NP_570116) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: MADCAM1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:4000
• Theoretical MW: 38.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Use in Porcine reported in scientific literature (PMID:32502552).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MAdCAM-1 Antibody (OTI1A5)

• hMAdCAM-1
• MACAM1
• MAdCAM1
• MAdCAM-1
• mucosal addressin cell adhesion molecule 1
• mucosal addressin cell adhesion molecule-1
• mucosal vascular addressin cell adhesion molecule 1

Background

Mucosal vascular addressin-1 (MAdCAM-1) is a type I transmembrane glycoprotein expressed at high levels on high endothelial venules (HEV) of Peyer's patches and mesenteric lymph nodes, and on flat walled venules within the gut lamina propria. It is also expressed on sinus-lining cells in the spleen. (1-3) The countereceptor or "homing receptor" for MAdCAM-1 is alpha4beta7 integrin (also known as LPAM-1). MAdCAM-1 is also a facultative ligand for CD62L/L-selectin. (4, 5) The monoclonal antibody MECA-367 binds to the first domain of MAdCAM-1 and blocks MAdCAM-1-dependent binding in vitro and lymphocyte homing to Peyer's patch HEV in vivo. (1, 2, 4)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MAdCAM-1 Antibody (NBP2-45706)(1)

Publication using NBP2-45706

• Jung K, Saif L, Wang Q Porcine epidemic diarrhea virus (PEDV): An update on etiology, transmission, pathogenesis, and prevention and control. Virus Res 2020-06-02 [PMID: 32502552] (ICC/IF, Porcine)

Review for MAdCAM-1 Antibody (NBP2-45706) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Porcine.

reviewed by: Verified Customer IHC-P Porcine 06/19/2019

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Mammary gland tissue
• Species: Porcine
• Lot: A001

Comments

• Comments: Antigen retrieval by a microwave method in 0.01M citric acid (pH 6.0). Alex Fluor 488 anti-mouse antibody was used as a secondary detection antibody.

Bioinformatics

• Gene Symbol: MADCAM1