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HAF019). A specific band was detected for MAdCAM-1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the endothelial cells of blood capillaries in intestinal villi. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox" target="_blank">
993-MC) was serially diluted and captured by Rat Anti-Mouse MAdCAM‑1 Monoclonal Antibody (Catalog # MAB9931) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse MAdCAM‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF993) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
HAF018). A specific band was detected for MAdCAM‑1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox" target="_blank">
MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." class="big_lightbox" target="_blank">
Species: Hu Applications: WB, Flow, CyTOF-ready Host: Mouse Monoclonal
Mucosal vascular addressin-1 (MAdCAM-1) is a type I transmembrane glycoprotein expressed at high levels on high endothelial venules (HEV) of Peyer's patches and mesenteric lymph nodes, and on flat walled venules within the gut lamina propria. It is also expressed on sinus-lining cells in the spleen. (1-3) The countereceptor or "homing receptor" for MAdCAM-1 is alpha4beta7 integrin (also known as LPAM-1). MAdCAM-1 is also a facultative ligand for CD62L/L-selectin. (4, 5) The monoclonal antibody MECA-367 binds to the first domain of MAdCAM-1 and blocks MAdCAM-1-dependent binding in vitro and lymphocyte homing to Peyer's patch HEV in vivo. (1, 2, 4)
![Western Blot MAdCAM-1 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/MAdCAM1_AF993_Western_Blot_17234.jpg)
![Western Blot MAdCAM-1 Antibody (683715) [Unconjugated]](http://images.novusbio.com/images/antibody/mab6056_human-madcam-1-mab-clone-683715-western-blot-1182021114949.jpg)
![N/A MAdCAM-1 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_MAdCAM1_DY605605_ELISA_2487.jpg)
![N/A MAdCAM-1 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_MAdCAM1_DY2768_ELISA_1884.jpg)
![Immunohistochemistry MAdCAM-1 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/MAdCAM-1_AF993_Immunohistochemistry_7223.jpg)
![ELISA MAdCAM-1 Antibody [Unconjugated]](http://images.novusbio.com/images/af993_mouse-madcam-1-affinity-purified-polyclonal-ab-elisa-639201854769196329.jpg)
![Flow Cytometry MAdCAM-1 Antibody (683715) [Unconjugated]](http://images.novusbio.com/images/antibody/MAdCAM-1_MAB6056_Flow_Cytometry_11194.jpg)
![Binding Activity MAdCAM-1 [Biotin]](http://images.novusbio.com/images/avi11379_recombinant-human-madcam-1-fc-chimera-avi-tag-protein-cf-binding-activity-45202314853..jpg)
![SDS-Page MAdCAM-1 [Biotin]](http://images.novusbio.com/fullsize/avi11379_recombinant-human-madcam-1-fc-chimera-avi-tag-protein-cf-sds-page-452023145127..jpg)
