Recombinant Rat M-CSF Animal-Free Protein Summary
An un-tagged bioactive recombinant protein corresponding to Rat M-CSF
Source: E. coli
Amino Acid Sequence: MEVSEHCSHM IGNGHLQILQ QLIDSQMETA CLIEYKFVDQ EQLDDPVCYL KKAFVLVQVI IEETMRFKDN TPNANATERL QELSMKLNSC FIKDYKEQNE ACVQTYKESP LRLLEKIKNF FNETKNFLEK DWNIFSKNCN DSLAKCSSRD VVTKP
Produced with no materials of animal or human origin using ANIMAL-FREE purification processes.
Production and Purification
- No animal-derived ingredients, No FBS
- E. coli based production system
- Animal-Free facility, fermentor, columns, filters, labware, lyophilization, and storage
- Endotoxin levels at <1 EUs/ug
- Purity levels at 95% or greater
M-CSF is a dimer, 18.1/36.2 kDa (155/210 aa)
Protein is derived from genetically modified E. coli.
|Details of Functionality
NFS-60 cell proliferation, >1.0 x 10^5 units/mg (ED50 acceptance criteria are <10 ng/mL).
| Protein/Peptide Type
>95%, by SDS-PAGE
Acceptance criteria for endotoxin levels are <1 EUs/ug protein (determined by Kinetic LAL Method).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at -20 to -80C. Avoid freeze-thaw cycles.
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5
>95%, by SDS-PAGE
Sterile water at 0.1 mg/mL
Lot Specific Information:
Given endotoxin and ED50 values are acceptance criteria standards. For lot specific values, please contact technical support.
Centrifuge vial before opening. Suspend the product by gently pipetting the recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots.
12 months from date of receipt when stored at -20C to-80C as supplied. 1 month when stored at 4C after reconstituting as directed. 3 months when stored at -20C to -80C after reconstituting as directed.
Alternate Names for Recombinant Rat M-CSF Animal-Free Protein
- colony stimulating factor 1 (macrophage)
- macrophage colony stimulating factor
- macrophage colony-stimulating factor 1
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed
for 3 months from date of receipt.
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