LYVE-1 Antibody [Alexa Fluor® 488]

• Reactivity: Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 488

LYVE-1 Antibody [Alexa Fluor® 488] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant rat LYVE-1
  Thr53-Thr259
  Accession # NP_001099756
• Specificity: Detects rat LYVE-1 in direct ELISA and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse LYVE-1 is observed, and approximately 10% cross-reactivity with recombinant human LYVE-1 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LYVE-1 Antibody [Alexa Fluor® 488]

• cell surface retention sequence binding protein-1
• Cell surface retention sequence-binding protein 1
• CRSBP1
• CRSBP-1
• extracellular link domain containing 1
• extracellular link domain-containing 1
• Extracellular link domain-containing protein 1
• HAR
• Hyaluronic acid receptor
• lymphatic vessel endothelial hyaluronan receptor 1
• lymphatic vessel endothelial hyaluronic acid receptor 1
• LYVE1
• LYVE-1
• UNQ230/PRO263
• XLKD1

Background

LYVE-1 (Lymphatic vessel endothelial hyaluronic acid receptor 1; also CRSBP-1) is a 58-64 kDa, monomeric, glycoprotein member of the Link protein superfamily of hyaluron-binding molecules. It has limited expression, being found on the cell surface of lymphatic endothelial cells, endothelial cells of lymphoid sinuses, nodal stromal cells, and macrophages plus dendritic cells. HA (hyaluronan) is a nonsulfated, freestanding, repeating disaccharide consisting of GlcA (glucuronic acid) in beta ‑linkage with GlcNAc (N-acetylglucosamine). It should not be confused with heparan, which is sulfated, protein-linked, and composed of repeating GlcA/IdoA and GlcNAc residues in both alpha - and beta -linkages. HA is ubiquitous and occupies space between collagen fibers. It undergoes both normal, and pathology-induced turnover, and presumably does so by binding to LYVE-1, CD44 and HARE on lymphatic endothelium. Ultimately, HA is transported to the liver and nodes where it undergoes degradation. This may be necessary as low MW HA is proinflammatory. LYVE-1 is also a receptor for PDGF-BB and VEGF-A, and LYVE-1 ligation apparently induces endothelial cell contraction with the opening of intercellular junctions. Based on mouse, rat LYVE-1 is synthesized as a 343 amino acid (aa) type I transmembrane protein that contains a very long signal sequence (aa 1-52). The extracellular region is likely to be 182 aa in length (aa 53-234) and contain one Link domain (aa 62‑210). LYVE-12 is likely maintained in a default "off mode" by undergoing sialylation, possibly at Thr83. Over aa 53-259, rat LYVE-1 shares 82% and 60% aa sequence identity with mouse and human LYVE-1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.