Lysyl tRNA synthetase Antibody

Images

 
Western Blot: Lysyl tRNA synthetase Antibody [NBP1-32063] - Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Lysyl tRNA synthetase Antibody [NBP1-32063] - Paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: Lysyl tRNA synthetase Antibody [NBP1-32063] - Paraffin-embedded mouse colon. Lysyl-tRNA synthetase antibody dilution: 1:50
Immunohistochemistry-Paraffin: Lysyl tRNA synthetase Antibody [NBP1-32063] - Paraffin-embedded NCI-N87 xenograft, using antibody at 1:100 dilution.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
2 mg/ml

Order Details

Lysyl tRNA synthetase Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Lysyl-tRNA synthetase. The exact sequence is proprietary.
Localization
Cytoplasm
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KARS
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Zebrafish (84%), Xenopus laevis (87%), Chicken (85%), Rhesus Monkey (96%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 40% Glycerol
Preservative
0.01% Thimerosal
Concentration
2 mg/ml
Purity
Protein A purified

Alternate Names for Lysyl tRNA synthetase Antibody

  • CMTRIB
  • EC 6.1.1
  • EC 6.1.1.6
  • KARS2
  • KIAA0070
  • KRS
  • lysine tRNA ligase
  • Lysine--tRNA ligase
  • lysRS
  • lysyl-tRNA synthetase

Background

lysyl-tRNA synthetase. Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol KARS