Lysyl tRNA synthetase Antibody - BSA Free

Western Blot: Lysyl tRNA synthetase Antibody [NBP1-32063] - Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE; antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: Lysyl tRNA synthetase Antibody [NBP1-32063] - Paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: Lysyl tRNA synthetase Antibody [NBP1-32063] - Paraffin-embedded mouse colon. Lysyl-tRNA synthetase antibody dilution: 1:50

Immunohistochemistry-Paraffin: Lysyl tRNA synthetase Antibody [NBP1-32063] - Paraffin-embedded NCI-N87 xenograft, using antibody at 1:100 dilution.

• Reactivity: Hu, Mu, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Lysyl tRNA synthetase Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Lysyl tRNA synthetase. The exact sequence is proprietary.
• Localization: Cytoplasm
• Predicted Species: Rhesus Macaque (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KARS1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 68 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (84%), Xenopus laevis (87%), Chicken (85%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 40% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Protein A purified

Alternate Names for Lysyl tRNA synthetase Antibody - BSA Free

• CMTRIB
• EC 6.1.1
• EC 6.1.1.6
• KARS2
• KIAA0070
• KRS
• lysine tRNA ligase
• Lysine--tRNA ligase
• lysRS
• lysyl-tRNA synthetase

Background

lysyl-tRNA synthetase. Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: KARS1