Lysosomal Pro-X Carboxypeptidase/PRCP Antibody

Images

 
Western Blot: Lysosomal Pro-X Carboxypeptidase/PRCP Antibody [H00005547-B01P] - PRCP MaxPab polyclonal antibody. Western Blot analysis of PRCP expression in human kidney.
Western Blot: Lysosomal Pro-X Carboxypeptidase/PRCP Antibody [H00005547-B01P] - Analysis of PRCP expression in transfected 293T cell line by PRCP polyclonal antibody. Lane 1: PRCP transfected lysate(54.56 KDa). Lane 2: ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Lysosomal Pro-X Carboxypeptidase/PRCP Antibody Summary

Immunogen
PRCP (NP_005031.1, 1 a.a. - 496 a.a.) full-length human protein. MGRRALLLLLLSFLAPWATIALRPALRALGSLHLPTNPTSLPAVAKNYSVLYFQQKVDHFGFNTVKTFNQRYLVADKYWKKNGGSILFYTGNEGDIIWFCNNTGFMWDVAEELKAMLVFAEHRYYGESLPFGDNSFKDSRHLNFLTSEQALADFAELIKHLKRTIPGAENQPVIAIGGSYGGMLAAWFRMKYPHMVVGALAASAPIWQFEDLVPCGVFMKIVTTDFRKSGPHCSESIHRSWDAINRLSNTGSGLQWLTGALHLCSPLTSQDIQHLKDWISETWVNLAMVDYPYASNFLQPLPAWPIKVVCQYLKNPNVSDSLLLQNIFQALNVYYNYSGQVKCLNISETATSSLGTLGWSYQACTEVVMPFCTNGVDDMFEPHSWNLKELSDDCFQQWGVRPRPSWITTMYGGKNISSHTNIVFSNGELDPWSGGGVTKDITDTLVAVTISEGAHHLDLRTKNALDPMSVLLARSLEVRHMKNWIRDFYDSAGKQH
Specificity
PRCP - prolylcarboxypeptidase (angiotensinase C),
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Gene
PRCP
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control.
Reviewed Applications
Read 1 Review rated 3
using
H00005547-B01P in the following application:

Reactivity Notes

Rat (kidney tissue type) reactivity reported from customer review.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

Quality control test: Antibody reactive against mammalian transfected lysate.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Lysosomal Pro-X Carboxypeptidase/PRCP Antibody

  • Angiotensinase C
  • EC 3.4.16.2
  • HUMPCP
  • Lysosomal carboxypeptidase C
  • lysosomal Pro-X carboxypeptidase
  • PCPMGC2202
  • PRCP
  • Proline carboxypeptidase
  • prolylcarboxypeptidase (angiotensinase C)
  • prolylcarboxypeptidase isoform 1 preproprotein
  • Prolylcarboxypeptidase

Background

The protein encoded by this gene is a lysosomal prolylcarboxypeptidase, which cleaves C-terminal amino acids linked to proline in peptides such as angiotension II, III and des-Arg9-bradykinin. The cleavage occurs at acidic pH, but the enzyme activity is retained with some substrates at neutral pH. This enzyme has been shown to be an activator of the cell matrix-associated prekallikrein. The importance of angiotension II, one of the substrates of this enzyme, in regulating blood pressure and electrolyte balance suggests that this gene may be related to essential hypertension. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lysosomal Pro-X Carboxypeptidase/PRCP Antibody (H00005547-B01P) (0)

There are no publications for Lysosomal Pro-X Carboxypeptidase/PRCP Antibody (H00005547-B01P).
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Review for Lysosomal Pro-X Carboxypeptidase/PRCP Antibody (H00005547-B01P) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using H00005547-B01P:
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WB
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Images Ratings Applications Species Date Details
Western Blot Lysosomal Pro-X Carboxypeptidase/PRCP H00005547-B01P
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reviewed by:
Anonymous
WB Rat 03/08/2010
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Summary

ApplicationWestern Blot
Sample TestedRat kidney tissue, Sample Amount: 156.8, 112.5, 90, 56.25, 45 ug
SpeciesRat
Lot08329
CommentsThe bottom image is the one I have given details for. The samples and molecular mass markers do not appear to be aligned properly. Therefore, we did not trust the apparent molecular mass of the sample.

Blocking

Blocking DetailsBlocking Buffer: 5%Non-Fat Dry Milk, Blocking Time: 1.5 hour, Blocking Temp: 23 degrees Celsius

Primary Anitbody

Dilution RatioDilution Ratio:1/500, Incubation Dilution Buffer:1% Non-Fat Dry Milk in 1xPBS w/ 0.1% Tween-20, Incubation Time/Temp:1 hr @ 23 C

Secondary Antibody

Secondary DescriptionSecondary Ab: Pierce ImmunoPure, Secondary Ab Dilution Ratio: 1/1000

Details

Detection NotesDetection Method: Chemiluminescence, Exposure Time: 30 sec, Specific Bands: ~107 kDa and 94 kDa, Non-Specific Bands: ~28 kDa

Comments

CommentsThe bottom image is the one I have given details for. The samples and molecular mass markers do not appear to be aligned properly. Therefore, we did not trust the apparent molecular mass of the sample.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Lysosomal Pro-X Carboxypeptidase/PRCP Antibody (H00005547-B01P) (0)

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Secondary Antibodies

 

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Recent Reviews

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Anonymous
03/08/2010
Application: WB
Species: Rat

Bioinformatics

Gene Symbol PRCP
Entrez
Uniprot