Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [Alexa Fluor® 750]

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 750

Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant human KDM4A
  Lys386-Ser514
  Accession # AAH02558
• Specificity: Detects human KDM4A in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human KDM4C is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [Alexa Fluor® 750]

• EC 1.14.11
• EC 1.14.11.-
• JHDM3A
• JHDM3AJMJD2
• JmjC domain-containing histone demethylation protein 3A
• JMJD2
• JMJD2A
• JMJD2Alysine-specific demethylase 4A
• jumonji domain containing 2
• jumonji domain containing 2A
• Jumonji domain-containing protein 2A
• KDM4A
• KIAA0677jumonji C domain-containing histone demethylase 3A
• Lysine (K)specific Demethylase 4A
• Lysine (K)-specific Demethylase 4A

Background

KDM4A (Lysine [K]-specific demethylase 4A; also known as JMJD2A) is a 155-160 kDa member of the JHDM3 histone demethylase family of enzymes. It is ubiquitously expressed and interacts with the histone deacetylase proteins HDAC-1, -2, and -3. KDM4A specifically demethylates trimethylated lysines on histone H3 at Lys9 and Lys36. Transcriptional repression occurs when it interacts with NCOR1. Human KDM4A is 1064 amino acids (aa) in length (SwissProt #:O75164). It contains one jumonji N domain (aa 13-55), a catalytic jumonji C domain (aa 175-291), two PHD zinc finger regions (aa 709-855), and two TUDOR methylated-histone binding domains (aa 897-1011). Over aa 386-514, human KDM4A shares 93% amino acid sequence identity with mouse KDM4A.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.