Rat LYAG/GAA ELISA Kit (Colorimetric)

ELISA: Rat LYAG/GAA ELISA Kit (Colorimetric) [NBP2-82445] - Samples were spiked with high concentrations of Rat LYAG/GAA and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat LYAG/GAA ELISA Kit (Colorimetric) [NBP2-82445] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 1.56 - 100 ng/mL
• Sensitivity: 0.94 ng/mL

Rat LYAG/GAA ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Rat LYAG/GAA in samples. No Significant cross-reactivity or interference between Rat LYAG/GAA and analogues was observed.
• Standard Curve Range: 1.56 - 100 ng/mL
• Sensitivity: 0.94 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.32
• Intra-Assay: CV% < 4.78
• Spike Recovery: 84-108%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: GAA

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat LYAG/GAA ELISA Kit (Colorimetric)

• Acid alpha-Glucosidase
• Acid Maltase
• Aglucosidase alfa
• EC 3.2.1.20
• GAA
• glucosidase, alpha; acid
• LYAG
• Lysosomal alphaGlucosidase
• Lysosomal alpha-Glucosidase

Background

GAA encodes acid alpha-glucosidase, which is essential for the degradation of glycogen to glucose in lysosomes. Different forms of acid alpha-glucosidase are obtained by proteolytic processing. Defects in this gene are the cause of glycogen storage disease II, also known as Pompe's disease, which is an autosomal recessive disorder with a broad clinical spectrum. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for LYAG/GAA ELISA Kit (NBP2-82445). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: GAA