Ly6K Antibody

Images

 
Western Blot: Ly6K Antibody [NBP2-24405] - Analysis using Ly6K protien with GST-tag, non-transfected control in HEK293T cells, HEK293T cells transfected with Ly6K-GFP contruct. Image from a verified customer review.
Genetic Strategies: Western Blot: Ly6K Antibody [NBP2-24405] - Analysis of Ly-6K in T24 bladder carcinoma cells using NBP2-24405. A. Ly-6K expression was seen in Ly-6K transfected (T24/Ly-6K-TF) but not ...read more
Genetic Strategies: Western Blot: Ly6K Antibody [NBP2-24405] - Analysis of Ly-6K in KK47 human bladder carcinoma cells using NBP2-24405. LY-6K siRNA, but not scrambled siRNA knocked down Ly-6K expression. A GAPDH ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

Ly6K Antibody Summary

Immunogen
Reacts with residues 82-100 RQRDPEDSQRTDEGDNRVW of the 14 kDa human Ly-6K protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LY6K
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA 1:100-1:2000
Application Notes
WB reported in a verified customer review.
Control
Ly6K Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
NBP2-24405 in the following applications:

Publications
Read Publication using NBP2-24405.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Ly6K Antibody

  • cancer/testis antigen 97
  • CO16
  • CT97
  • FLJ35226
  • HSJ001348
  • Ly6K
  • ly-6K
  • lymphocyte antigen 6 complex, locus K
  • lymphocyte antigen 6K

Background

To identify novel human head-and-neck squamous cell carcinoma (HNSCC)-associated Ly-6 members with squamous cell-associated expression patterns, a comprehensive gene-screening was performed, followed by expression analysis. Using this approach, the Ly-6K gene was identified as a novel member of the human Ly-6 family. Results show that Ly-6K is a potential target antigen for the diagnosis and therapy of HNSCC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ly6K Antibody (NBP2-24405)(1)

Review for Ly6K Antibody (NBP2-24405) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-24405:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Ly6K NBP2-24405
Enlarge
5
reviewed by:
Anonymous
WB Human 06/09/2016
View

Summary

ApplicationWestern Blot
Sample Testedoverexpressed Ly6K in HEK293T cell lysate and reconbinant Ly6K
SpeciesHuman
LotR611

Blocking

Blocking Details1% BSA in PBS buffer

Primary Anitbody

Dilution Ratio1:1000 in 2% BSA-PBS overnight at 4C

Secondary Antibody

Secondary Descriptiongoat anti-rabbit IgG
Secondary Manufacturer Cat#cellsignaling #7074
Secondary Concentration1:5000

Details

Detection Notesincubate with ECL for 5 min and 1 min exposure.

Comments

CommentsThis antibody works very well with over-expressed and recombinant Ly6K, very strong bands and very little background, almost no nonspecific bands in my western blot.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Ly6K Antibody (NBP2-24405) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics Tool for Ly6K Antibody (NBP2-24405)

Discover related pathways, diseases and genes to Ly6K Antibody (NBP2-24405). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Ly6K

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Recent Reviews

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1
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Anonymous
06/09/2016
Application: WB
Species: Human

Bioinformatics

Gene Symbol LY6K