Ly-6C Antibody (ER-MP20) [Janelia Fluor® 525]

• Reactivity: Mu
• Applications: Flow, IHC, IP
• Clone: ER-MP20
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Janelia Fluor 525

Ly-6C Antibody (ER-MP20) [Janelia Fluor® 525] Summary

• Immunogen: Balb/c macrophage precursor cell hybrids
• Specificity: Ly6C (ER-MP20)
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: Ly6c1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Ly-6C Antibody (ER-MP20) [Janelia Fluor® 525]

• Ly 6c
• Ly6c protein
• Lymphocyte antigen 6 complex locus C
• Lymphocyte antigen 6 complex
• Lymphocyte antigen 6C
• Lymphocyte antigen Ly 6C

Background

Ly-6C is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T-cell subsets. Mice with the Ly-6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly-6C+ and CD8+Ly-6C+ cells, while Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly-6C+ lymphocytes. Ly-6C may play a role in the development and maturation of lymphocytes. (1-4)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Ly6c1