LXR beta/NR1H2 Antibody

Immunocytochemistry/Immunofluorescence: LXR beta/NR1H2 Antibody [NB100-1464] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Flow Cytometry: LXR beta/NR1H2 Antibody [NB100-1464] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: ELISA, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

LXR beta/NR1H2 Antibody Summary

• Immunogen: Peptide with sequence SSPTTSSLDTPLPGC corresponding to N-Terminus according to NP_009052.3.
• Specificity: Based on the peptide used, this antibody is expected to be specific for LXR beta and not to cross-react with LXR alpha.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NR1H2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:28000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LXR beta/NR1H2 Antibody

• Liver X receptor beta
• LXR beta
• LXR-b
• NER-I
• NERliver X receptor-beta
• NR1H2
• nuclear orphan receptor LXR-beta
• Nuclear receptor NER
• Nuclear receptor subfamily 1 group H member 2
• nuclear receptor subfamily 1, group H, member 2
• oxysterols receptor LXR-beta
• RIP15
• steroid hormone-nuclear receptor NER
• Ubiquitously-expressed nuclear receptorLXRB
• UNR
• UNRLX receptor beta

Background

Liver X Receptors (LXRs) are oxysterol activated nuclear receptors which are involved in the regulation of cholesterol metabolism and bile acids. Two known isoforms, alpha and beta, are differentially expressed. The alpha isoform is predominantly expressed in liver, whereas the beta isoform expression has been shown to be ubiquitous.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LXR beta/NR1H2 Antibody (NB100-1464)(4)

Publications using NB100-1464

• Wong, KW;Zeng, Y;Tay, E;Teo, JHJ;Cipta, NO;Hamashima, K;Yi, Y;Liu, H;Warrier, T;Le, MTN;Ng, SC;Li, QJ;Li, H;Loh, YH; Nuclear receptor-SINE B1 network modulates expanded pluripotency in blastoids and blastocysts Nature communications 2024-11-19 [PMID: 39562549]
• Chen Y, Xu Y, Zhao H, Zhou Y et Al. Myeloid-derived suppressor cells deficient in cholesterol biosynthesis promote tumor immune evasion Cancer Lett 2023-05-07 [PMID: 37150500]
• Mo J, Fang SJ, Chen W et al. Regulation of ALK-1 signaling by the nuclear receptor LXRbeta. J Biol Chem 2002-12-01 [PMID: 12393874]
• Tangirala RK, Bischoff ED, Joseph SB et al. Identification of macrophage liver X receptors as inhibitors of atherosclerosis. Proc Natl Acad Sci U S A 2002-09-03 [PMID: 12193651]

Bioinformatics

• Gene Symbol: NR1H2
• Entrez:
  • 7376(Human)
• Uniprot:
  • P55055(Human)