Lubricin/PRG4 Antibody

Immunohistochemistry-Paraffin: Lubricin/PRG4 Antibody [NBP1-89984] - Staining of human rectum shows strong cytoplasmic and nuclear positivity in glandular cells.

Product Discontinued

Lubricin/PRG4 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: IGPSQTHTIRIQYSPARLAYQDKGVLHNEVKVSILWRGLPNVVTSAISLPNIRKPDGYDYYAFSKDQYYNIDVPSRTARAITTRSGQTLSKVWYNC
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRG4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (85%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Lubricin/PRG4 Antibody

• bG174L6.2 (MSF: megakaryocyte stimulating factor )
• bG174L6.2
• CACP
• FLJ32635
• HAPO
• Hemangiopoietin
• JCAP
• Lubricin
• megakaryocyte stimulating factor
• Megakaryocyte-stimulating factor
• MSF
• MSFproteoglycan 4, (megakaryocyte stimulating factor, articular superficial zoneprotein, camptodactyly, arthropathy, coxa vara, pericarditis syndrome)
• PRG4
• Proteoglycan 4
• Superficial zone proteoglycan
• SZP
• SZPHAPOarticular superficial zone protein

Background

Lubricin is a glycoprotein encoded by the PRG4 gene. It has been identified as a component of joint lubrication and synovial homeostasis by reducing friction in joints and preventing synovial cell overgrowth. It is a large, water soluble molecule that acts as a carrier for insoluble surface-active phospholipid (SAPL). Depletion of lubricin function has been associated with camptodactyly-arthropathy-coxa vara-pericarditis syndrome (CACP), an arthritic-like autosomal recessive disorder.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRG4