LTK Antibody

Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human LTK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4664) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LTK at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

LTK was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Sheep Anti-Human LTK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4664) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hemotoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for LTK at approximately 77 kDa (as indicated) using 50 µg/mL of Sheep Anti-Human LTK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4664) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Product Discontinued

LTK Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human LTK
  Ser10-Pro424
  Accession # P29376
• Specificity: Detects human LTK in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: LTK
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 50 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LTK Antibody

• EC 2.7.10.1
• leukocyte receptor tyrosine kinase
• leukocyte tyrosine kinase receptor
• leukocyte tyrosine kinase
• LTK
• TYK1
• TYK1Protein tyrosine kinase 1

Background

LTK (Leukocyte tyrosine kinase; also protein tyrosine kinase 1) is a glycoprotein member of the tyrosine protein kinase family, insulin receptor subfamily of proteins. There are multiple isoforms that run between 50 and 100 kDa in SDS-Page. It is reportedly expressed in lymphocytes and cerebral cortex neurons, plus cardiomyocytes where it plays a role in cellular hypertrophy. Mature human LTK is an 848 amino acid (aa) type I transmembrane protein that is embedded in the ER. It contains a 408 aa extracellular domain (aa 17-424) plus a 415 aa cytoplasmic region (aa 450-864). The cytoplasmic region possesses multiple phosphotyrosines that interact with downstream signaling molecules. There is also a protein kinase domain between aa 510-786. LTK apparently undergoes dimer- and trimerization under certain circumstances. There are multiple splice variants for LTK. Two isoforms possess a common deletion of aa 274-334 that may be accompanied by a 27 aa substitution for aa 449-544. Another isoform shows an alternative start site at Met406 accompanied by a 17 aa substitution for Val449, while a fourth isoform contains a 51 aa substitution for aa 171-864. A final 50 kDa isoform shows a 28 aa substitution for aa 449-864. Over aa 17-424, human LTK shares 75% aa identity with mouse LTK.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LTK
• Uniprot:
  • P29376()