LSM4 Antibody - BSA Free

Western Blot: LSM4 Antibody [NBP3-04445] - Analysis of 200ug extracts of Jurkat cells using LSM4 antibody . Western blot was performed from the immunoprecipitate using this antibody.

Western Blot: LSM4 Antibody - BSA Free [NBP3-04445] - Western blot analysis of various lysates, using LSM4 Rabbit pAb (A5891) at 1:1500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

LSM4 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit LSM4 Antibody - BSA Free (NBP3-04445) is a polyclonal antibody validated for use in WB, ELISA and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-139 of human LSM4 (NP_036453.1). MLPLSLLKTAQNHPMLVELKNGETYNGHLVSCDNWMNINLREVICTSRDGDKFWRMPECYIRGSTIKYLRIPDEIIDMVKEEVVAKGRGRGGLQQQKQQKGRGMGGAGRGVFGGRGRGGIPGTGRGQPEKKPGRQAGKQ
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LSM4
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 ug/mL
  • Immunoprecipitation 0.5μg-4μg antibody for 200ug-400ug extracts of whole cells
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 15 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for LSM4 Antibody - BSA Free

• Glycine-rich protein
• GRP
• LSM4 homolog, U6 small nuclear RNA associated (S. cerevisiae)
• U6 snRNA-associated Sm-like protein LSm4
• YER112W

Background

Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family. Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LSM4