LRRTM3 Antibody [Unconjugated]

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LRRTM3 was detected in immersion fixed frozen sections of human hippocampus using Human LRRTM3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4898) at 15 µg/mL overnight at 4 °C. Tissue was stained using ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

LRRTM3 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human LRRTM3
Glu31-Lys419
Accession # Q86VH5
Specificity
Detects human and mouse LRRTM3 in direct ELISAs and Western blots. In direct ELISA, approximately 25% cross-reactivity with recombinant human (rh) LRRTM4, and less than 5% cross-reactivity with rhLRRTM1 and rhLRRTM2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
LRRTM3
Endotoxin Note
<2.5 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LRRTM3 Antibody [Unconjugated]

  • leucine rich repeat transmembrane neuronal 3
  • leucine-rich repeat transmembrane neuronal protein 3
  • LRRTM3
  • MGC131810

Background

Human LRRTM3 (leucine-rich repeat transmembrane neuronal 3) is a 63 kDa (predicted) type I transmembrane protein, and one of four members of the LRRTM family of proteins within the leucine-rich repeat (LRR) superfamily (1). There are two isoforms of LRRTM3. Isoform 1 is synthesized as a precursor with a 30 amino acid (aa) signal sequence, a 389 aa extracellular region, a 21 aa transmembrane region, and a 141 aa cytoplasmic region. In isoform 2, aa 513 is changed from isoleucine to valine, and aa 514 to 581 are missing, producing a cytoplasmic region of only 74 aa. The extracellular region of both isoforms contains one N-linked glycosylation site, a leucine-rich repeat N-terminal domain bordered by four conserved cysteines, and 10 LRRs flanked by cysteine-rich domains (1). The cytoplasmic region of both isoforms contains several tyrosine, serine, and threonine residues that have the potential to be phosphorylated, and thus to be involved in signal transduction (1). The C-terminal of isoform 2 also contains a conserved glutamic acid-cysteine-glutamic acid-valine sequence for potential interaction with PDZ proteins (1, 2). Mature human LRRTM3 (isoform 1) is 98% identical to mouse LRRTM3. In the mouse, beginning at 8.5 dpc, strong levels of LRRTM3 can be detected in the neural progenitors of the neural plate that will develop into the rostral neural tube, the forebrain, a stripe in the hindbrain, and the region of the presomitic mesoderm/somite boundary (2). By 15 dpc, LRRTM3 is expressed broadly and accumulates thereafter (3). In the adult, LRRTM3 is expressed almost exclusively in the brain with high expression in the cortical laminae and dentate gyrus, as well as detectable levels in the hypothalamus and amygdala (3). Functionally, LRRTM3 may be involved in the formation of the CNS and maintenance of CNS structure and function in the adult brain (1). In addition, LRRTM3 has been shown to promote processing of amyloid-precursor protein by BACE1, and is a positional candidate gene for late-onset Alzheimer’s disease (3).

  1. Lauren, J. et al. (2003) Genomics 81:411.
  2. Haines, B.P. and P.W.J. Rigby (2007) Gene Expr. Patterns 7:23.
  3. Majercak, J. et al. (2006) Proc. Natl. Acad. U.S.A. 103:17967.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol LRRTM3
Entrez
Uniprot