LRRTM4 Antibody

Western blot shows lysates of human brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human LRRTM4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5377) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for LRRTM4 at approximately 65 and 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

LRRTM4 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human LRRTM4
  Gln31-Lys424
  Accession # Q86VH4
• Specificity: Detects human LRRTM4 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human (rh) LRRTM1, rhLRRTM2, and rhLRRTM3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: LRRTM4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LRRTM4 Antibody

• FLJ12568
• leucine rich repeat transmembrane neuronal 4
• leucine-rich repeat transmembrane neuronal 4 protein
• leucine-rich repeat transmembrane neuronal protein 4
• LRRTM4
• MGC120633
• MGC120636

Background

LRRTM4 (leucine rich repeat transmembrane protein 4) is a 59kDa (predicted) and 67 kDa (predicted) glycoprotein, member of the LRRTM family of proteins. All members of this family are type I transmembrane proteins that contain 10 external LRR repeats plus a short cytoplasmic tail that binds PDF motifs. LRRTM4 is found in the neurons of the cerebral cortex, dentate gyrus granule cells, and cerebellar Purkinje cells. Mature human LRRTM4 is 560 amino acids (aa) in length. The extracellular region contains one Cys‑rich N-terminal flanking domain (aa 33‑59), multiple LRRs (aa 60‑299), and a Cys‑rich C-terminal flanking domain (aa 306‑363). There is one splice variant that shows a Val substitution for cytoplasmic aa 518‑590. Over aa 31‑424, human LRRTM4 shares 95% aa identity with mouse LRRTM4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LRRTM4 Antibody (AF5377)(2)

Publications using AF5377

• Melina A. Agosto, Theodore G. Wensel LRRTM4 is a member of the transsynaptic complex between rod photoreceptors and bipolar cells Journal of Comparative Neurology 2021-01-01 [PMID: 32390181]
• Melina A. Agosto, Theodore G. Wensel LRRTM4 is a member of the transsynaptic complex between rod photoreceptors and bipolar cells Journal of Comparative Neurology 2021-01-01 [PMID: 32390181] (Immunohistochemistry, Mouse)

Review for LRRTM4 Antibody (AF5377) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IHC-Fr Mouse 07/14/2020

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: C57BL/6 mouse retina
• Species: Mouse
• Lot: CCAP021812A

Bioinformatics

• Gene Symbol: LRRTM4
• Uniprot:
  • Q86VH4()