LRRTM1 Antibody

Western blot shows lysates of SH-SY5Y human neuroblastoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human LRRTM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4897) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LRRTM1 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

LRRTM1 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human LRRTM1
  Ala35-Ile425
  Accession # Q86UE6
• Specificity: Detects human LRRTM1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross reactivity with recombinant human (rh) LRRTM2 is observed, and less than 5% cross reactivity rhLRRTM3 and rhLRRTM4 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: LRRTM1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LRRTM1 Antibody

• FLJ32082
• leucine rich repeat transmembrane neuronal 1
• leucine-rich repeat transmembrane neuronal 1 protein
• leucine-rich repeat transmembrane neuronal protein 1
• LRRTM1

Background

Human LRRTM1 (leucine-rich repeat transmembrane neuronal 1) is a 58‑59 kDa (predicted) type I transmembrane protein, that belongs to the LRRTM family of proteins within the leucine-rich repeat (LRR) superfamily (1). It is synthesized as a precursor with a 34 amino acid (aa) signal sequence, a 393 aa luminal region, a 21 aa transmembrane region, and a 74 aa cytoplasmic region. The luminal portion of LRRTM1 contains three N-linked glycosylation sites and 10 LRRs flanked by cysteine-rich domains (1). The cytoplasmic region contains several tyrosine, serine, and threonine residues that have potential to be phosphorylated and thus to be involved in signal transduction (1). The C-terminal also contains a conserved glutamic acid-cysteine-glutamic acid-valine sequence for potential interaction with PDZ proteins (1‑2). Mature human LRRTM1 is 97% aa identical to mouse LRRTM1. LRRTM1 is localized to the endoplasmic reticulum (3). In the mouse, beginning at 9dpc, low levels of LRRTM1 can be detected in the overlying ectoderm of the limb bud, dorsal otic vesicle, forebrain, midbrain, hindbrain, and in neural progenitors in the central neural tube (2). In the adult brain, it is highly expressed in the brain and salivary gland, and is detected at intermediate levels in the cerebellum, spinal chord, stomach, testis, and uterus (1). Functionally, LRRTM1 may be involved in the formation of the CNS and maintenance of CNS structure and function in the adult brain (1). In addition, LRRTM1 has been shown to be a maternally suppressed gene that is associated paternally with handedness and schizophrenia (3).

1. Lauren, J. et al. (2003) Genomics 81:411.
2. Haines, B.P. and P.W.J. Rigby (2007) Gene Expr. Patterns 7:23.
3. Francks, C. et al. (2007) Mol. Psychiatry 12:1129.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LRRTM1 Antibody (AF4897)(2)

Publications using AF4897

• Keary KM, Gu QH, Chen J, Li Z Dendritic distribution of autophagosomes underlies pathway-selective induction of LTD Cell reports 2023-07-28 [PMID: 37516958]
  
  Details:
  Dilutions: 1:500
• KP de Arce, A Ribic, D Chowdhury, K Watters, GJ Thompson, BG Sanganahal, ETC Lippard, A Rohlmann, SM Strittmatt, M Missler, F Hyder, T Biederer Concerted roles of LRRTM1 and SynCAM 1 in organizing prefrontal cortex synapses and cognitive functions Nature Communications, 2023-01-28;14(1):459. 2023-01-28 [PMID: 36709330] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: LRRTM1
• Uniprot:
  • Q86UE6()