LRRTM2 Antibody

LRRTM2 was detected in immersion fixed frozen sections of embryonic mouse neural tube (E10.5) using 10 µg/mL Sheep Anti-Human/Mouse LRRTM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5589) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Western blot shows lysates of human brain cortex and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse LRRTM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5589) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LRRTM2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

LRRTM2 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Anti-Human/Mouse LRRTM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5589) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

LRRTM2 was detected in immersion fixed frozen sections of mouse neural tube (E9.5) using Sheep Anti-Human/Mouse LRRTM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5589) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (yellow; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

LRRTM2 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human LRRTM2
  Cys34-Arg422
  Accession # O43300
• Specificity: Detects human and mouse LRRTM2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) LRRTM1, rhLRRTM3, and rhLRRTM4 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: LRRTM2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LRRTM2 Antibody

• leucine-rich repeat transmembrane neuronal protein 2
• leucine rich repeat transmembrane neuronal 2
• LRRN2
• LRRTM2

Background

LRRTM2 (leucine rich repeat transmembrane protein # 2) is a 59 kDa (predicted) member of the LRRTM family of proteins. All members of this family are type I transmembrane proteins that contain 10 external LRR repeats plus a short cytoplasmic tail that binds PDF motifs. LRRTM2 is found in deep neurons of the hippocampus (CA1-CA3), dentate gyrus granule cells, and cerebellar granular cells, and in almost all peripheral tissues at some level. Mature human LRRTM2 is 483 amino acids (aa) in length. The extracellular region contains ten LRRs (aa 61‑299), followed by a 73 aa cytoplasmic tail (aa 444‑516). Over aa 34‑422. human LRRTM2 shares 98% aa identity with mouse LRRTM2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LRRTM2 Antibody (AF5589)(3)

Publications using AF5589

• Liouta, K;Lubas, M;Venugopal, V;Chabbert, J;Jeannière, C;Diaz, C;Munier, M;Tessier, B;Claverol, S;Favereaux, A;Sainlos, M;de Wit, J;Letellier, M;Thoumine, O;Chamma, I; LRRTM2 controls presynapse nano-organization and AMPA receptor sub-positioning through Neurexin-binding interface Nature communications 2024-10-11 [PMID: 39394199] (Neutralization, Immunocytochemistry, Transgenic Mouse)
• Nuria Gresa-Arribas, Jesús Planagumà, Mar Petit-Pedrol, Izumi Kawachi, Shinichi Katada, Carol A. Glaser et al. Human neurexin-3 alpha antibodies associate with encephalitis and alter synapse development Neurology 2016-06-14 [PMID: 27170573]
• Hayer S, Bading H Nuclear calcium signaling induces expression of the synaptic organizers Lrrtm1 and Lrrtm2. J Biol Chem, 2014-12-19;290(9):5523-32. 2014-12-19 [PMID: 25527504] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: LRRTM2
• Uniprot:
  • O43300()