LRRFIP1 Antibody

Product Discontinued

LRRFIP1 Antibody Summary

• Immunogen: Antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human LRRFIP1. The immunogen is located within the last 50 amino acids of LRRFIP1.
• Specificity: LRRFIP1 antibody is predicted to not cross-react with other LRRFIP family members. Three isoforms of LRRFIP1 are known to exist; this antibody will only recognize the two longer isoforms.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LRRFIP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LRRFIP1 Antibody

• FLAP-1
• FLIIAP1
• GC-binding factor 2
• GCF2
• GCF-2
• HUFI-1
• leucine rich repeat (in FLII) interacting protein 1
• leucine-rich repeat flightless-interacting protein 1
• LRR FLII-interacting protein 1
• MGC10947
• MGC119738
• MGC119739
• NEDD8-conjugating enzyme
• TRIPTAR RNA-interacting protein

Background

LRRFIP1, also known as GC-binding factor 2 (GCF2), is a 738 amino acid transcriptional repressor that mainly plays a cytoskeletal role. It is primarily localized in the cytoplasm and preferentially binds to GC-rich dsDNA, but will also bind directly to dsRNA as well. The RNA binding domain encompasses a lysine-rich motif. LRRFIP1 interacts with the mammalian Flightless I (Fli-I) and is a key component in the cytoskeletal regulation of platelet function. LRRFIP1 and the related protein LRRFIP2 may modulate canonical WNT signaling and mediate the IRF3-induced production of type I interferon via a beta-catenin-dependent pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LRRFIP1
• Uniprot:
  • NP_001131022(Human)