LRRC4 Antibody (701419) [Alexa Fluor® 350]

• Applications: IHC
• Clone: 701419
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

LRRC4 Antibody (701419) [Alexa Fluor® 350] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human LRRC4
  Ala39-Lys527
  Accession # Q9HBW1
• Specificity: Detects human LRRC4 in direct ELISAs. In direct ELISAs, greater than 25% cross-reactivity with recombinant human (rh) NGL-1/LRRC4C is observed, and no cross-reactivity with rhLRRC4B is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

LRRC4 (Leucine rich repeat/LRR-containing protein 4), also called NGL-2 (netrin-G ligand-2) or NAG14 (nasopharyngeal carcinoma-associated gene 14) is a 55 kDa (predicted, unglycosylated) type I transmembrane protein that is a member of the NGL family of synaptic LRR adhesion molecules (1, 2). Human LRRC4 cDNA encodes 653 amino acids (aa) that include a 38 aa signal sequence, a 489 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 105 aa cytoplasmic domain. The ECD contains nine LRRs (aa 74‑288), a C2 type Ig like domain (aa 354‑440), and a Thr-rich segment (aa 455‑526). Within the ECD, human LRRC4 shares 98% aa identity with mouse and rat, 99% aa identity with canine and bovine, and 99.6% aa identity with equine LRRC4. It also shares 54‑55% aa identity with family members LRRC4C/NGL-1 and LRRC4B/NGL-3, but each recognizes different ligands (1). LRRC4 is predominantly expressed in the brain on neurons and astrocytes as a ligand for netrin-G2 on the dendritic surface of synaptic neurons (2-4). It is proposed to regulate the formation of excitatory synapses via recruitment of PSD-95 to the cytoplasmic domain after aggregation of LRRC4 at the surface (3, 5). It suppresses proliferation by downregulating cell signaling pathways, resulting in altered expression of cell cycle regulating proteins and delay at the late G1 phase (1, 2, 6-8). It is thus considered a tumor suppressor protein and is often downregulated in brain tumors, particularly gliomas (1, 2, 6). Forced expression of LRRC4 in tumor cells slows proliferation and promotes differentiation (1, 4, 9). Addition of soluble LRRC4 to cultured neurons reduces excitatory synapse formation (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.