LRRC32/GARP Antibody (GARP5) - BSA Free

Western Blot: Mouse Monoclonal LRRC32/GARP Antibody (GARP5) [NBP2-37710} - Western blotting analysis was performed on RIPA buffer extracts of thrombocytes, leukocytes, and Jurkat cells mixed with hot reducing SDS-loading buffer. Samples were resolved using 10% SDS-PAGE gel.Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody GARP5 (2 ug/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). Mouse anti-GAPDH monoclonal antibody FF26A conjugated with DyLight 680 (0.1 ug/ml), was used as the loading control (red). Multiplex fluorescent Western blot detection was performed.GARP was detected at ~80 kDa in thrombocytes.

• Reactivity: Hu
• Applications: WB, Flow
• Clone: GARP5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

LRRC32/GARP Antibody (GARP5) - BSA Free Summary

• Description: Novus Biologicals Mouse LRRC32/GARP Antibody (GARP5) - BSA Free (NBP2-37710) is a monoclonal antibody validated for use in WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified human sGARP protein.
• Specificity: Antibody recognizes GARP / LRRC32, an approximately 80 kDa glycoprotein expressed e.g. on the surface of megakaryocytes, platelets and activated Treg cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LRRC32
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Western Blot 1-2 ug/ml
• Application Notes: Western Blot: reducing conditions
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for LRRC32/GARP Antibody (GARP5) - BSA Free

• D11S833E
• D11S833Eleucine-rich repeat-containing protein 32
• GARP
• GARPGarpin
• Garpin
• Glycoprotein A repetitions predominantgarpin
• leucine rich repeat containing 32
• LRRC32

Background

LRRC32 encodes a type I membrane protein which contains 20 leucine-rich repeats. Alterations in the chromosomal region 11q13-11q14 are involved in several pathologies. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same isoform.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LRRC32