LRP-4 Antibody (S207-27) - BSA Free

Western Blot: LRP-4 Antibody (S207-27) [NBP2-22416] - Western Blot analysis of Rat brain membrane lysate showing detection of LRP-4 protein using Mouse Anti-LRP-4 Monoclonal Antibody, Clone S207-27 (NBP2-22416). Primary Antibody: Mouse Anti-LRP-4 Monoclonal Antibody (NBP2-22416) at 1:500, 1:1000, and 1:2000.

Immunocytochemistry/Immunofluorescence: LRP-4 Antibody (S207-27) [NBP2-22416] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-LRP-4 Monoclonal Antibody, Clone S207-27 (NBP2-22416). Tissue: COS cells transfected with 2 ug human LRP-4 plasmid. Species: Human. Fixation: 4% PFA for 10 min. Primary Antibody: Mouse Anti-LRP-4 Monoclonal Antibody (NBP2-22416) at 1:100 for 1 hour at RT. Secondary Antibody: Goat anti-mouse: Alexa 488. Counterstain: DAPI.

Immunocytochemistry/Immunofluorescence: LRP-4 Antibody (S207-27) [NBP2-22416] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-LRP4 (Extracellular) Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X.

Immunocytochemistry/Immunofluorescence: LRP-4 Antibody (S207-27) [NBP2-22416] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-LRP-4 (Extracellular) Monoclonal Antibody, Clone S207-27 (NBP2-22416). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-LRP-4 (Extracellular) Monoclonal Antibody (NBP2-22416) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) LRP-4 (Extracellular) Antibody (D) Composite.

Immunocytochemistry/Immunofluorescence: LRP-4 Antibody (S207-27) [NBP2-22416] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-LRP-4 (Extracellular) Monoclonal Antibody, Clone S207-27 (NBP2-22416). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-LRP-4 (Extracellular) Monoclonal Antibody (NBP2-22416) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) LRP-4 (Extracellular) Antibody. (D) Composite.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: S207-27
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

LRP-4 Antibody (S207-27) - BSA Free Summary

• Immunogen: Fusion protein amino acids 26- 350 (extracellular N-terminus) of mouse LTP4
• Localization: Membrane
• Specificity: Detects the extracellular domain of LRP4 approx 215kDa. Degradation product can be seen at approx 150kDa.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: LRP4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000
• Application Notes: 1 ug/ml of LRP4 (Extracellular) Antibody was sufficient for detection of LRP4 in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for LRP-4 Antibody (S207-27) - BSA Free

• CLSS
• KIAA0816
• low density lipoprotein receptor-related protein 4
• low-density lipoprotein receptor-related protein 4
• LRP10
• LRP4
• LRP-4
• MEGF7LRP10
• Multiple epidermal growth factor-like domains 7

Background

Potential cell surface endocytic receptor, which binds and internalizes extracellular ligands for degradation by lysosomes

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LRP4
• Uniprot:
  • Q8VI56()